OBJECTIVE: To investigate the characteristics of material and energy metabolism of 800 meters race, to improve the scientific training of the 800 meters race and to verify the feasibility of nuclear magnetic resonance-based urine metabonomics in sports training monitoring.

METHODS: Seven middle distance athletes were subject to an 800 meters race at ultimate intensity, and their urine metabonomics analysis was conducted in combination with the Human Metabolism Database and other databases before and after exercise.

RESULTS AND CONCLUSION: OPLS-DA model showed that the metabolic samples before and after movement presented the cluster-like distribution. The change was mainly caused by the following metabolites, including leucine, lactic acid, creatinine, succinic acid, hypoxanthine, and adenosine. Through the analysis we could infer that the 800 meters race is a sport event mainly dependent on glycolysis for energy supply, working together with raw phosphate and aerobic oxidation for energy supply. The amino acid metabolism and methane metabolism of the athletes were more vigorous after 800 meters running, and moreover, there were certain oxidative stress during the 800 meters race. These findings indicate that nuclear magnetic resonance-based urine metabonomics takes the advantages of invasive, high sensitivity, less sample requirement, simple processing.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程