Expression of hypoxia inducible factor-1 alpha in human lumbar nucleus pulposus of different herniated types

doi:10.3969/j.issn.2095-4344.2015.29.021

Abstract

Abstract:

BACKGROUND: Under hypoxic environment, hypoxia inducible factor-1 plays an important role in regulation of hypoxia-induced gene expression in the intervertebral disc. Hypoxia-inducible factor-1 consists of α and β subunits, and which hypoxia inducible factor-1α determines the stability and activity of hypoxia-inducible factor-1.
OBJECTIVE: To observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus of different herniated types and to judge their relationships.
METHODS: A total of 60 nucleus pulposus samples were harvested from the lumbar vertebra, including 41 from L4-5 and 19 from L5-S1, and then divided into protruded group and sequestered group, with 30 cases in each group. Meanwhile, another 10 samples of lumbar nucleus pulposus served as controls. Hematoxylin-eosin staining and streptavidin-biotin peroxidase complex immunohistochemical technique were used to observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus in different groups.
RESULTS AND CONCLUSION: The expression level of hypoxia inducible factor-1α was (58.2±7.5)% in the sequestered group, (27.3±2.3)% in the protruded group, and (10.5±4.7)% in the control group, which was significantly higher in the sequestered group than the other two groups (P < 0.01). These findings indicate that the expression of hypoxia inducible factor-1α in the lumbar nucleus pulposus is associated with the herniated types, which is the highest in the prolapse sequestered type.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Intervertebral Disk Displacement, Spinal Cord, Hypoxia-Inducible Factor 1

CLC Number:

R318

Liu Xing-zhen, Fu Zhi-yi, Shen Kang-ping, Jin Wen-jie, Wu Yu-jie. Expression of hypoxia inducible factor-1 alpha in human lumbar nucleus pulposus of different herniated types [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(29): 4700-4709.

Figures/Tables 2

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