Application of patch-clamp technique in traditional Chinese medicine: a visual analysis of relevant literature

doi:10.12307/2026.316

Abstract

Abstract: BACKGROUND: In recent years, the interdisciplinary application potential of patch-clamp technique in traditional Chinese medicine research has gradually emerged, but a systematic summary of its applications in this field has not yet been conducted.
OBJECTIVE: To visualize the application of patch-clamp technology in traditional Chinese medicine field through CiteSpace knowledge map analysis, and to reveal the progress and trends of this technology in the modernization research of traditional Chinese medicine.
METHODS: The literature sources included CNKI, VIP, WanFang, PubMed and Web of Science Core Collection database. The computer-assisted literature search was conducted to build a database of patch-clamp technology applications in traditional Chinese medicine field from database inception to September 2024. The authors, institutions, and keywords were subjected to visual analysis and knowledge map drawing using CiteSpace 6.3.R1 software and bibliometric methods.
RESULTS AND CONCLUSION: (1) A total of 819 articles were included, with 968 authors. The First Affiliated Hospital of Henan University of Chinese Medicine was the institution with the most publications in the Chinese database, while Harbin Medical University was the institution with the most publications in the English database. (2) The research directions, keyword clustering, and emergence analysis of each institution showed that patch-clamp technology in the field of traditional Chinese medicine mainly focused on cardiac electrophysiology, herbal pharmacology, and nervous system electrophysiology. (3) The development of patch-clamp technology in traditional Chinese medicine generally presents the evolution path of “basic mechanism → target deepening → clinical transformation,” reflecting the transformation from a single technical tool to a multidisciplinary cross-platform. (4) The core influence of the authors who published the articles needs to be further improved, and the cross-regional cooperation among research institutions needs to be enhanced. The current research relies on a single technical approach and lacks depth in content. Future research should integrate multiple technologies and interdisciplinary approaches to enrich the research content, expand research directions, and provide evidence-based support for in-depth exploration of the intrinsic mechanisms of traditional Chinese medicine.

Key words: traditional Chinese medicine, patch clamp, CiteSpace, electroacupuncture, neurophysiology, cardiovascular electrophysiology, Chinese medicine pharmacology

CLC Number:

Yang Jun, Li Bin, Xing Guogang, Cai Jie, Liu Lu, Chen Peng, Zhang Tao, Fu Yuanbo, Liu Huilin. Application of patch-clamp technique in traditional Chinese medicine: a visual analysis of relevant literature[J]. Chinese Journal of Tissue Engineering Research, 2026, 30(23): 6010-6020.

Figures/Tables 9

2.2 作者可视化分析作者可视化分析可对研究领域内合作模式、学术影响力的深度解析，通过发节点大小(通常基于发文量或被引频次)和中介中心性，可快速定位领域内的高影响力学者。若某作者节点较大且中介中心性高，表明其不仅是高产作者，还可能是连接不同研究团队的桥梁。通过节点(作者)与连线(合作次数)的可视化，展示学术合作合作网络与团队结构。纳入此次研究的819篇文献中，共包含作者968名。依据普莱斯定律计算得出该领域研究的中英文核心作者的最低发文量约为2篇，核心作者共计129位(中文46，英文83)[9]，发文量≥4篇的核心作者有17位(中文3，英文14)。将纳入文献绘制中英文文献作者共现图谱(图3)，中文文献共计节点493个，37条网络连线，图谱的网络密度为0.000 3，作者之间未形成核心作者群及合作关系图中显示核心研究力量不足，研究作者较为分散，没有形成广泛合作的网络系统，整体结构分散。中文文献发文量最多为中国中医科学院西苑医院刘建勋、北京中医药大学刘洪珍。英文文献共计节点475个，976条网络连线，图谱的网络密度为0.008 7，发文量最高(7篇)为中国科学院上海生命科学研究院Shi Yu Liang。英文文献的个别核心作者在小范围构建了合作，河北医科大学Chu L与河北中医药大学Zhang Y，Zhang X合作较为紧密。 2.3 机构可视化分析机构可视化分析可了解机构合作网络、发现核心机构、了解学科交叉情况，通过节点大小快速定位领域内高产机构，连线的粗细或颜色反映机构间合作频次。对纳入研究的机构进行可视化分析如图4所示，中文文献发文排名前5分别为河南中医药大学第一附属医院(19篇)、武汉大学人民医院(17篇)、中国医科大学(11篇)、北京中医药大学(11篇)、中南民族大学(8篇)、中国中医科学院(8篇)。英文文献发文排名前3为哈尔滨医科大学(8篇)、河北医科大学(7篇)、釜山大学(7篇)。涉及膜片钳在中医药领域研究的机构较多，且机构之间联系较为广泛。中文文献中北京中医药大学的研究机构中具有广泛的合作关系，拥有10个连接点，显示了其显著的影响力和学术网络中的核心地位；其次为中国中医科学院(7个连接点)、武汉大学人民医院(7个连接点)。英文文献中机构影响力排名前3为南京中医药大学(10个连接点)、釜山大学(9个连接点)、河北医科大学(8个连接点)。 2.4 关键词可视化分析关键词共现图谱可直观显示该领域研究热点，图谱中节点的大小反映关键词出现的频率，连线的紧密程度反映节点中介中心性大小，关键词的中介中心性越大提示其在共现网络中的影响力越大。研究纳入819篇国内外相关文献提取关键词，将同义词合并，绘制关键词共现图谱(图5)，中文文献共计506个节点，1 383条连线，网络密度为0.010 8，英文文献共计467个节点，891条线，网络密度为0.008 2。将关键词归纳为干预方式、研究对象、研究内容、离子通道4类，以出现频次为据分类排序，见表1。中文文献以“中药”及“电针”为热点治疗措施，“丹参”在中药研究中尤为关注。“心律失常” “动作电位”及“镇痛”为主要研究内容，关注对象集中为“心肌细胞” “海马”，关注离子通道主要涉及“L-型钙离子通道电流” “大电导钙激活钾通道”及“钾通道”。英文文献与中文文献治疗措施大体相似，以“中药” “丹参”为主。研究对象存在差异，英文文献主要关注对象为“Cajal间质"

细胞” “背根神经节”，研究热点集中于“动作电位” “神经病理痛”及“胃肠道”，离子通道主要围绕“钙通道”及“钾通道”展开。关键词聚类分析将具有相似研究主题的文献进行聚类，形成不同的研究主题群，从而帮助研究者了解某一领域的研究动态和发展趋势。在图5的基础上，选择对数似然比算法对聚类的关键词进行标记，得到关键词的聚类图谱(图6)，中文数据库共形成506个节点，1 383条连线，12个聚类模块，聚类模块Q值=0.619 6> 0.3，S=0.880 7> 0.5，英文数据库共形成467个节点，891条连线，12个聚类模块，聚类模块Q值=0.845 2> 0.3，S=0.957 5> 0.5，中英文数据库聚类结构合理，具有高度可信性。中文数据库聚类：#0、#2、#5、#12为研究内容；#1、#3、#4、#6、#8、#10为干预措施；#9、#11为研究离子通道，研究主要涉及中医药效应的体液调节及中药安全性，电针镇痛机制及调节突触可塑性的效应机制。英文数据库关键词聚类归纳：#0、#1、#2、#10为干预措施， #6、#8、#9、#11为研究内容，#3、#4为研究对象，#5、#7为离子通道，研究关注中药单体或复方心脑血管保护中的作用，电针神经调节机制，提示钙通道及钠离子调节是各种效应机制发挥的靶点。时线图展示研究主题的时间演变，峰峦图可识别研究热点的突变，将两者结合可有助于研究者了解某一领域的研究动态和发展趋势。将中英文数据库的关键词聚类标签绘制时间线图及峰峦图，图7，8显示膜片钳技术在中医药领域发展过程中#0、#1、#2 持续受到学者关注，自2008-2024年，#6、#7、#10的关注逐渐降低，近10来年(2014-2024)更多的研究聚焦于#4、#5、#12也是日后发展的趋势。图9，10显示英文数据库的文献中关于#1、#2、#3研究自2000年持续至今，近10年#0、#4、#5、#6、#7、#8、#9热度下降，#10新近研究方向。 2.5 关键词突现分析突现分析是指分析某一时间段内关键词使用频次的显著增加，从而识别研究领域的热点、前沿和动态变化。将纳入中英文数据库文献绘制关键词突线图谱，见图11。中文数据库自1990-2009年，“钙通道”和“心肌细胞”是研究的热点；2010年至现阶段，“电针”和“镇痛”成为了新的研究焦点。自2018年来，对电针关注持续升温，同时间段“镇痛”研究尤为突出。2020年后突现的“突触可塑性”持续时间短，有一定的发展空间。英文数据库关键词如“patch clamp”(1992年)、“calcium current”(1995年)和“calcium channel”(2004年)等，在特定年份开始出现突现，持续时间近20年(1992-2013年)，研究者对该领域拥有持续的兴趣，2021年以来，“neuropathic pain”为新的关注热点。"

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