Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (22): 3480-3486.doi: 10.12307/2022.274
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Liu Ming, Wang Kai
Received:
2020-12-29
Revised:
2021-01-26
Accepted:
2021-06-06
Online:
2022-08-08
Published:
2022-01-11
Contact:
Wang Kai, Chief physician, First Department of Orthopedics, Qinghai Provincial People’s Hospital, Xining 810000, Qinghai Province, China
About author:
Liu Ming, Master, Associate chief physician, First Department of Orthopedics, Qinghai Provincial People’s Hospital, Xining 810000, Qinghai Province, China
CLC Number:
Liu Ming, Wang Kai. Theaflavin-3-gallate modified nano-hydroxyapatite/polycaprolactone composite porous scaffold in bone defect repair[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(22): 3480-3486.
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2.1 TF-3G的细胞毒性 培养72 h后CCK-8检测结果显示,0.86,4.30,8.60,17.20,34.4,68.8,86,106,126,146 mg/L TF-3G溶液组的相对细胞增殖率分别为95.35%,107.35%,118.09%,125.34%,110.72%,93.27%,88.79%,74.13%,68.13%,59.23%,显示0.86-86 mg/L TF-3G溶液的毒性分级为0级,106 mg/L TF-3G溶液的毒性为1级,而126,146 mg/L TF-3G溶液的细胞毒性为2级;阴性对照组的相对细胞增殖率为98.62%,阳性对照组为11.38%,说明此次实验结果符合细胞毒性反应国家分级标准。 2.2 TF-3G对细胞增殖活性的影响 培养第1天时,各组之间细胞增殖活性比较差异无显著性意义(P > 0.05);培养第3天时,34.4,68.8,86,106 mg/L组的细胞增殖活性低于0 mg/L组(P < 0.05),其余各浓度组细胞增殖活性与0 mg/L组比较差异无显著性意义(P > 0.05);培养第5天时,4.30,8.60 mg/L组的细胞增殖活性高于0 mg/L浓度组(P < 0.05),17.20,34.4,68.8,86,106 mg/L组的细胞增殖活性低于0 mg/L组(P < 0.05),0.86 mg/L组的增殖活性与0 mg/L组比较差异无显著性意义(P > 0.05),并且8.60 mg/L组细胞增殖活性高于0.86,4.30 mg/L组(P < 0.05);培养第7天时,0.86,4.30,8.60 mg/L组的细胞增殖活性高于0 mg/L组(P < 0.05),17.20,34.4,68.8,86,106 mg/L组的细胞增殖活性低于0 mg/L组(P < 0.05),并且8.60 mg/L组的细胞增殖活性高于0.86,4.30 mg/L组(P < 0.05),见表3。各组细胞增殖情况见图1。"
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