Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (22): 3466-3472.doi: 10.3969/j.issn.2095-4344.3177
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Liu Jun1, 2, 3, Yang Long1, Wang Weiyu1, 2, 3, Zhou Yuhu1, 2, 3, Wu Ying3, 4, Lu Tao3, Shu Liping2, 3, Ma Minxian4, Ye Chuan1, 2, 3
Received:
2020-04-18
Revised:
2020-04-23
Accepted:
2020-06-17
Online:
2021-08-08
Published:
2021-01-19
Contact:
Ye Chuan, Chief physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, and Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:
Liu Jun, Master candidate, Physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, and Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
Supported by:
CLC Number:
Liu Jun, Yang Long, Wang Weiyu, Zhou Yuhu, Wu Ying, Lu Tao, Shu Liping, Ma Minxian, Ye Chuan. Preparation and properties of poly3-hydroxybutyrate 4-hydroxybutyrate/polyethylene glycol/graphene oxide tissue-engineered scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3466-3472.
2.5.3 细胞Live/Dead染色结果 共培养第7天的扫描电镜显示,P34HB组细胞呈球状分布于纤维丝,未出现明显的细胞铺展及伪足伸出;P34HB/PEG组细胞与纤维丝融合,但数量较少;P34HB/PEG/GO组细胞铺展,细胞与纤维丝融合,伪足伸出,见图8。共培养第7天的Live/Dead染色显示,P34HB组出现大量红色荧光(死亡细胞),P34HB/PEG及P34HB/PEG/GO组红色荧光明显减少,见图8。经image pro plus 6.0计算,P34HB组、P34HB/PEG组、P34HB/PEG/GO组的细胞存活率分别为(41.14±3.11)%,(81.21±2.50)%,(93.25±5.21)%。"
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