Fetal bovine serum exosomes promote the proliferation of osteoblasts

doi:10.3969/j.issn.2095-4344.2122

Abstract

Abstract:

BACKGROUND: Exosomes have been shown to promote bone regeneration, but whether extracting exosomes from fetal bovine serum can promote osteogenesis remains controversial.

OBJECTIVE: To observe the effect of fetal bovine serum exosomes on the proliferation of osteoblasts, so as to provide new ideas for treating bone destruction.

METHODS: Exosomes were extracted from fetal bovine serum by ultracentrifugation method. Electron transmission microscopy and western blot assay were used to verify whether the exosomes were successfully extracted. MC3T3-E1 cells were interfered with 10 mg/L fetal bovine serum. The effect of exosomes on the proliferation of osteoblasts was detected by cell counting kit-8 assay. The effects of exosomes on the expression of osteogenic markers bone morphogenetic protein-2 and osteopontin protein were detected by western blot assay. MC3T3-E1 cells cultured in the fetal bovine serum without exosomes were as control group.

RESUITS AND CONCLUSION: The typical lipid bilayer membrane structure of exosomes was observed, and the size was between 30-150 nm. The exosome surface marker factor CD81 was positive, while the microcapsule surface marker CD40 was negative. The results of cell counting kit-8 assay showed that the proliferative capacity in the exosomes group was significantly higher than that in the control group (P < 0.05). The expression levels of bone markers bone morphogenetic protein-2 and osteopontin in the exosome group were significantly higher than those in the control group (P < 0.05). The exosomes extracted from fetal bovine serum can promote the proliferation of osteoblasts, providing a new idea for the clinical treatment of bone destruction.

Key words: exosomes, fetal bovine serum, osteogenic precursor cells, bone destruction, bone morphogenetic protein-2, osteopontin, CD81, CD40

CLC Number:

Xu Huijun, Zhang Mi, Shi Dongmei, Wu Saixuan, Lu Ying, Dong Ming, Niu Weidong. Fetal bovine serum exosomes promote the proliferation of osteoblasts [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 4961-4965.

Figures/Tables 4

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