Exosomes from adipose-derived stem cells can promote cavernous nerve regeneration in rats

doi:10.3969/j.issn.2095-4344.2121

Abstract

Abstract:

BACKGROUND: Cavernous nerve is one of the main nerves regulating penile erection, which is easy to be damaged in pelvis surgery and due to trauma and induce erectile dysfunction. The direct effect of stem cell-derived exosome on the regeneration of injured cavernous nerve has not been reported yet.

OBJECTIVE: To observe the effect of the exosomes from adipose-derived stem cells on the regeneration of cavernous nerve.

METHODS: Three-dimensional culture model of rat nerve tissue in vitro was constructed. After 24 hours of incubation, the experimental group was incubated in the RPMI-1640 medium containing penicillin-streptomycin and exosomes (10¹⁵/L) from adipose-derived stem cells. The control group was incubated in the RPMI-1640 medium. The length of axon regeneration at the incisal edge of the cavernous nerve was measured to evaluate the regeneration ability of the cavernous nerve at 2, 5 and 9 days after intervention.

RESULTS AND CONCLUSION: Adipose-derived stem cells extracted from rat perirenal adipose tissue adhered with stable morphology and could be induced to differentiate into adipose, bone and cartilage. The exosomes extracted from the passage 3 adipose-derived stem cells showed similar vesicle structure with a diameter near 85 nm. In the three-dimensional culture system, the regeneration ability of the cavernous nerve itself was certain. The axon regeneration length in the experimental group was significantly longer than that in the control group at 2, 5 and 9 days after intervention. The exosomes from adipose-derived stem cells directly act on the cavernous nerve and have a significant promoting effect on nerve regeneration.

Key words: adipose-derived stem cells, exosomes, cavernous nerve, major pelvic ganglion, axon, regeneration, three-dimensional culture

CLC Number:

Gu Yubo, Zhang Dongliang, Yuan Wei, , Song Lujie. Exosomes from adipose-derived stem cells can promote cavernous nerve regeneration in rats[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 4979-4985.

Figures/Tables 10

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