Effects of hyaluronic acid on scar formation in the acellular nerve allograft

doi:10.3969/j.issn.2095-4344.2016.42.013

Abstract

Abstract:

BACKGROUND: In terms of the histocompatibility, immune rejection and scar formation after repair, acellular nerve allograft is closer to autologous nerve cells. At present, hyaluronic acid has been applied for autologous peripheral nerve repair; however, research on the nerve allograft is rarely reported.
OBJECTIVE: To explore the effect of hyaluronic acid on the anastomotic scar in acellular nerve allograft repair of rat sciatic nerve defect.
METHODS: Thirty-six Sprague-Dawley rats were randomly divided into three groups (n=12 per group). The rat model of nerve defect of 10 mm was established by cutting the sciatic nerve of the left hind leg and then given nerve allograft combined with the injection of hyaluronic acid at anastomosis (experimental group), only nerve allograft (control group) and autologous nerve graft (nerve autograft group), respectively. Afterwards, the healing of the proximal anastomosis was observed and scar components were assessed.
RESULTS AND CONCLUSION: Gross observations showed that the rat skin and muscle fascia had no significant differences in healing among groups, while the surrounding tissue adhesion in the experimental group was milder than that in the control group (P < 0.05). Masson staining found that collagen deposition in the epinerium could be observed in each group. In the experimental group, a small amount of collagen fibers arranged orderly in the epineurium; in the control group numerous collagen fibers accumulated and arranged irregularly; in the nerve autograft group, sparse epineurial collagen fibers appeared in an order arrangement. The gray value of collagen type I in the experimental group was higher than that in the control group (P < 0.05), while the gray value of collagen type III was lower than that in the control group (P < 0.05). No significant differences were found in the sum gray values of collagen type I and III among groups (P > 0.05). These findings indicate that in the peripheral nerve repair, hyaluronic acid abrogates the scar formation by increasing the deposition of collagen type III and reducing the deposition of collagen type I.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hyaluronic Acid, Sciatic Nerve, Cicatrix, Collagen Type I, Collagen Type III

CLC Number:

R318

Liu Ying-wei, Zhang Wan-li, Chi Cheng-tao, Xu Qing-yu, Lu De-zhi. Effects of hyaluronic acid on scar formation in the acellular nerve allograft[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.42.013.

Figures/Tables 2

2.1 实验动物数量分析纳入36只SD大鼠，随机分为3组，每组12只。全部进入结果分析，无脱失。 2.2 术口大体观察所有大鼠麻醉下切开伤口观察神经吻合口与周围组织的粘连情况。术后各组中大部分SD大鼠皮肤及肌肉、筋膜无裂开，小部分SD大鼠皮肤及肌肉、筋膜部分裂开，无明显感染现象。将Peterson评分(数据结果为等级分类资料)按定量资料多重比较方差分析，除了实验组神经吻合口与周围组织粘连较轻，优于对照组之外(P < 0.05)，3组其他指标行多重比较，差异均无显著性意义(P > 0.05)。各组大体观察Peterson评分结果见表2及图1。 2.3 Masson染色 Masson染色后在400倍光镜下观察所见各组均可见神经吻合口外膜胶原沉积，图2A可见实验组神经外膜胶原纤维排列整齐有序，胶原纤维量略少。图2B可见对照组大量胶原纤维成堆积状，排列紊乱。图2C可见自体神经移植组神经外膜胶原纤维较多，胶原纤维排列较整齐，但胶原纤维较稀疏。可见实验组优于对照组及自体神经移植组。 2.4 Ⅰ，Ⅲ型胶原相对含量分析 Ⅰ型胶原灰度值实验组高于对照组(P < 0.05)，实验组与自体神经移植组相比较差异无显著性意义(P=0.256 > 0.05)，自体神经移植组高于对照组(P < 0.05)。 Ⅲ型胶原灰度值实验组低于对照组(P < 0.05)，实验组与自体神经移植组比较差异无显著性意义(P > 0.05)。对照组高于自体神经移植组(P < 0.05)。 Ⅰ型+Ⅲ型胶原灰度值实验组与对照组比较、实验组与自体神经移植组比较、对照组与自体神经移植组比较P分别为0.165，0.941，0.145，P均 > 0.05，差异无显著性意义。虽然3组Ⅰ型+Ⅲ型胶原总量差异无显著性性意义，但实验组Ⅲ型胶原较对照组含量高，而Ⅰ型胶原含量较对照组少，实验组明显优于对照组，与自体神经移植差异无显著性性意义。Ⅰ，Ⅲ型胶原免疫组化灰度测量结果见表3。"

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