Effect of breviscapine on protein kinase C-gamma expression in brain tissue of rat models of traumatic brain injury

doi:10.3969/j.issn.2095-4344.2015.49.010

Abstract

Abstract:

BACKGROUND: Preventing secondary injury after traumatic injury is of great importance, which is also the target of brain protective treatment.
OBJECTIVE: To preliminarily investigate the protective effect of breviscapine on brain tissue of rat models of traumatic brain injury through observing the effect of breviscapine on protein kinase C-γ expression in injured brain tissue.
METHODS: Sixty-five Sprague-Dawley rats were randomly divided into control (n=5), sham (n=20), model (n=20) and breviscapine (n=20) groups. Rat models of traumatic brain injury were established in the model and breviscapine groups by a falling heavy object. In the sham group, rat bone window was open through an incision made on the scalp, and then the incision was sutured immediately. Rats in the breviscapine group were intraperitoneally injected with 0.4 mg/kg breviscapine daily immediately after injury. Rats in the model and sham groups were intraperitoneally injected with 0.4 mL/kg normal saline. The expression of protein kinase C-γ protein in brain tissue was detected by immunohistochemistry.
RESULTS AND CONCLUSION: After traumatic brain injury, the protein expression of protein kinase C-γ was significantly increased (P < 0.05), and reached the peak at 24 hours. Breviscapine could significantly decrease the protein expression of protein kinase C-γ in brain tissue after traumatic brain injury (P < 0.05). These results demonstrate that breviscapine has a protective effect on brain tissue after traumatic brain injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Yin Yan-qing, Fang Qi, Liao Zhuang-bin, Lin Heng, Huang Zi-xiong, Liang Yuan-sheng, Xu Cheng-jie. Effect of breviscapine on protein kinase C-gamma expression in brain tissue of rat models of traumatic brain injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7926-7931.

Figures/Tables 2

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