Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (16): 2557-2562.doi: 10.3969/j.issn.2095-4344.2014.16.016

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Nano-hydroxyapatite/polycaprolactone electrospinning scaffolds repair bone defects around the immediate implant

Li Jia-feng1, Cui Qun2, Sun Xiu-ying2, Xu Lei2, Sun Jin-hu1, Han Jian-guo2   

  1. 1Department of Oral Maxillofacial Surgery, Affiliated Xuzhou City Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China; 2School of Stomatology, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China
  • Revised:2014-02-06 Online:2014-04-16 Published:2014-04-16
  • Contact: Li Jia-feng, Department of Oral Maxillofacial Surgery, Affiliated Xuzhou City Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • About author:Li Jia-feng, Master’s supervisor, Chief physician, Associate professor, Department of Oral Maxillofacial Surgery, Affiliated Xuzhou City Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • Supported by:

    the Social Development Plan of Xuzhou City, No. XF10C027

Abstract:

 BACKGROUND: Alveolar bone remodeling and sustained absorption due to tooth extraction seriously affect the implanting conditions and morphology of hard and soft tissue in implant zone.

OBJECTIVE: To evaluate the effect of nano-hydroxyapatite/polycaprolactone electrospinning scaffolds to improve the osteogenic effect of bone defects around immediate implants.
METHODS: Tissue-engineered bone was prepared by combining canine bone marrow mesenchymal stem cells with nano-hydroxyapatite/polycaprolactone electrospinning scaffold. Bilateral mandibular second premolars from six dogs were extracted mandibular second premolar, and an immediate implant was placed in the mesial fossa of the mandibular second premolar. Three-wall bone defects was made buccally using titanium nails, then tissue-engineered bone and Bio-Oss bone powders were implanted bilaterally covered by collagen membranes (Bio-Gide). Imageology examination was performed to measure bone gray levels immediately, 4, 8, 12 weeks after surgery. After 12 weeks, the mandible was removed completely, toluidine blue staining was used for observation of microstructure, new bone formation, bone morphology and implant osseointegration.
RESULTS AND CONCLUSION: Between the two groups, there was no difference in bone mineral density at each time point after surgery, indicating that the effects of the two materials to promote bone regeneration process are basically the same. After implantation, the dense lamellar bone formed in the bone defect region of tissue-engineered bone group, mature bone cells, Haversian canal, and implant osseointegration were visible. While, in the Bio-Oss group, the lamellar bone was dense, a small amount of Bio-Oss particles distributed within new bone tissues, fewer bone cells were found, a part of Haversian canal was shown to have blood capillaries, and new bone was in close conjunction with the implant. These findings indicate that the nano-hydroxyapatite/polycaprolactone electrospinning scaffold combined with bone marrow mesenchymal stem cells and Bio-Gide collagen membrane can promote the regeneration of alveolar bone around the implant.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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Key words:  biocompatible materials, dental implants, hydroxyapatites, nanoparticles

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