Cytotoxicity evaluation of nano-Ag-SiO2 urethral catheter for external urethral mucosa cells

doi:10.3969/j.issn.2095-4344.2014.16.015

Abstract

Abstract:

BACKGROUND: Nano-Ag is increasingly used as antibacterial materials in medical products due to its excellent antibacterial activity. Antibacterial activated nano-Ag-SiO₂ urethral catheter can reduce the incidence of catheter-associated urinarytract infections obviously. As more and more nano-Ag medical products have been developed, their biological security is becoming a focused issue.

OBJECTIVE: To evaluate the biosecurity of nano-Ag-SiO₂ urethral catheter through comparing the active influence of nano-Ag-SiO₂ urethral catheter and ordinary urethral catheter extracts on in vitro cells of human prostatic urethra epithelial cells in different periods.

METHODS: Mechanical separation and enzymatic digestion were used to isolate and culture urothelial mucosa of benign prostatic hyperplasia patients to make cell suspension after proliferation in vitro. The medium was replaced by nano-Ag-SiO₂ and general catheter tube extracts, respectively. MTT assay was performed to quantify the in vitro cytotoxicity of two extracts on human urethra epithelial cells which were cultured for 2, 5 and 7 days. Absorbance values of nano-Ag-SiO₂ and general urinary catheter groups were measured by enzyme-linked immunosorbent assay to calculate the relative growth rate, and toxicity evaluation was compared and evaluated. Under an inverted microscope, cell morphology, growth and proliferation were observed.

RESULTS AND CONCLUSION: The cytotoxicity of extract of nano-Ag-SiO₂ catheter was 1 grade, and that of general medical catheter extract was 0 to 1 grade. There was no statistical difference in group effect of relative proliferation rate (F=0.544, P=0.475); there was statistical difference in time effect of relative proliferation rate (F=3.031, P=0.086); no statistical difference was found in the interaction between group and time (F=0.130, P=0.879). These findings indicate that nano-Ag-SiO₂ catheter has no or low cytotoxicity effect on cell growth and proliferation, which is consistent with the biological evaluation standards of medical devices.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, nanoparticles, urothelium, silicon dioxide

CLC Number:

R318

Zhang Zhan, Liu Chun. Cytotoxicity evaluation of nano-Ag-SiO₂urethral catheter for external urethral mucosa cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(16): 2550-2556.

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References

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Cytotoxicity evaluation of nano-Ag-SiO₂urethral catheter for external urethral mucosa cells

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