Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1443-1446.doi: 10.3969/j.issn.1673-8225.2012.08.025

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Feasibility of cancellous bone matrix in periodontal tissue engineering

Lu Hong1, Tian Yu2, Wu Zhi-fen1   

  1. 1Department of Periodontology & Oral Medicine, 2Department of Dental Pulp Diseases, School of Stomatology, the Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China
  • Received:2011-10-01 Revised:2011-12-14 Online:2012-02-19 Published:2012-02-19
  • Contact: Wu Zhi-fen, Chief physician, Professor, Doctoral supervisor, Department of Periodontology & Oral Medicine, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • About author:Lu Hong☆, Doctor, Attending physician, Lecturer, Department of Department of Periodontology & Oral Medicine, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China lu_hong@yahoo.com Tian Yu☆, Doctor, Associate professor, Associate chief physician, Department of Dental Pulp Diseases, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China tianyu@fmmu.edu.cn
  • Supported by:

    Science and Technology Research and Development Program of Shaanxi Province, No. 2010K12-02-03*

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Abstract:

BACKGROUND: Cancellous bone matrix (CBM) has been proved to be valuable in the bone regeneration and bone tissue engineering based on its double characteristics of bone induction and bone conduction.
OBJECTIVE: To analyze the cytocompatibility and cytotoxicity of CBM in order to evaluate the feasibility of CBM application in periodontal tissue engineering.
METHODS: Human periodontal ligament cells (PDLCs) cultured with modified-tissue block method in vitro were collected and seeded on three-dimensional framework of CBM. The growth and adhesion of PDLCs on the scaffolds were observed by cell counting and scanning electronic microscope. And the influences of the CBM extracts on cell proliferation and differentiation were observed by MTT assay and alkaline phosphatase activity assay.
RESULTS AND CONCLUSION: Cells adhered and proliferated on the three-dimensional framework of CBM, and scanning electronic microscope showed the porous structure of CBM and eugonic growth of cells on the scaffolds. The differences in cell proliferation and differentiation of different concentrations of CBM extracts had no significance compared with the negative control group. It is indicated that CBM has a good three-dimensional structure and cytocompatibility and has no cytotoxicity, and CBM is expected to be used in periodontal tissue regeneration and engineering.

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