Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1354-1358.doi: 10.3969/j.issn.1673-8225.2012.08.006

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Transfection of early growth response gene-1 DNA enzyme mediated by nanoparticles inhibits vein graft intimal hyperplasia

Liu Cheng-wei1, Zhang Xue-song1, Wang Shi1, Wang Shu-qing1, Tian Hao1, Cheng Ming-xun1, Hu Xin-hua2, Xin Shi-jie2,  Duan Zhi-quan2   

  1. 1First Department of General Surgery, the First Affiliated Hospital of Jiamusi University, Jiamusi  154003, Heilongjiang Province, China; 2Department of Vascular Surgery, the First Affiliated Hospital of China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-08-03 Revised:2011-09-14 Online:2012-02-19 Published:2012-02-19
  • About author:Liu Cheng-wei☆, Doctor, Associate chief physician, First Department of General Surgery, the First Affiliated Hospital of Jiamusi University, Jiamusi 154003, Heilongjiang Province, China liucwcw@163.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30801123*, 30872527* 

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Abstract:

BACKGROUND: The method of gene has been admitted to prevent stenosis and occlusion of grafted vascular. But it is a hot spot of how to increase gene transfection efficiency.
OBJECTIVE: To study the effect of partial transfection of exogenous early growth response gene-1 DNA enzyme (Egr-1 DNA enzyme, EDRz) mediated by nanoparticles (NP) on the intimal hyperplasia (IH) in grafted vein.
METHODS: The EDRz was constructed. Nanoparticle EDRz complex was prepared with polylactic/poly glycolic acid (PLGA) and polyvinyl alcohol (PVA). Autogenously vein graft model was established, and the 210 rats were divided into three groups randomly: (1) EDRz group, EDRz mediated by NP was transfected into the veins before anastomosis. (2) Empty vector group, the vein was transfected by empty vector mediated by NP. (3) Control group, no transfection.
RESULTS AND CONCLUSION: In EDRz group, the expression of mRNA and protein of Egr-1 gene in intimal was significant decreased than that in other two groups (P < 0.05) and at the 7th, 14th and 28th days, the thickness of intimal hyperplasia was decreased than that in other two groups (P < 0.01), the apoptotic percentage of vascular smooth muscle cells in EDRz group was significant increased than that in other two groups (P < 0.05). EDRz expression can prevent intimal hyperplasia and promote apoptosis of vascular smooth muscle cells after autogenous vein grafting.

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