Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (53): 9960-9964.doi: 10.3969/j.issn.2095-4344.2012.53.016

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Expression of transforming growth factor-beta 1 in intestinal graft intervened by cyclosporine A

Jin Xiao-shi1, Jiao Zheng-wei1, Zhang Ai-min1, Cheng Shu-jie1, Liu Tong2, Wang Peng-zhi2   

  1. 1Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
    2General Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2012-05-12 Revised:2012-07-10 Online:2012-12-30 Published:2012-12-30
  • About author:Jin Xiao-shi☆, Doctor, Associate chief physician, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China doctorjinxiaoshi@126.com

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Abstract:

BACKGROUND: The chronic rejection of intestinal graft is the important factor affecting the long term prognosis.
OBJECTIVE: To observe the effect of cyclosporine A on the expression of transforming growth factor-β1 in intestinal graft in rats.
METHODS: Allogeneic small intestine transplantation was performed in rats by microsurgical technology with the donor of F344(RT11vr) rats and the recipient of Lewis(RT11) rats. The graft biopsy and routine pathologic examination were performed at 7, 28 and 60 days after modeling. mRNA transcription level of transforming growth factor-β1 was detected by real time-PCR. Immunohistochemical staining was also performed to detect the expression of transforming growth factor-β1.
RESULTS AND CONCLUSION: The mRNA transcription level of transforming growth factor-β1 was significantly decreased during the application of cyclosporine A at 7-28 days after modeling. At 28-60 days after modeling, the mRNA transcription level was significantly increased after termination of cyclosporine A. Cyclosporine A can inhibit the expression of transforming growth factor-β1 in allogeneic small intestine transplantation.

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