Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 818-822.doi: 10.3969/j.issn.1673-8225.2012.05.014

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Specificity of new quantitative monitoring of immune status in skin transplantation model rats

Pan Ming-xin, Zhong Li-min, Wang Yan, Gao Yi, Jiang Ze-sheng   

  1. Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-12-08 Revised:2012-01-17 Online:2012-01-29 Published:2012-01-29
  • Contact: Jiang Ze-sheng, Doctor, Associate chief physician, Associate professor, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China sjiang@hotmail.com Gao Yi, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Pan Ming-xin☆, Doctor, Chief physician, Professor, Doctoral supervisor, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China mxwxy@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No.30972825*

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Abstract:

BACKGROUND: How to monitor the recipient’s immune status, and predict the occurrence of rejection after organ transplantation, in order to adjust the dosage of immunosuppressive agents is the important issue faced currently.
OBJECTIVE: To investigate antigen-specificity of new quantitative monitoring of immune status in skin transplantation model rats
METHODS: The C57→BALB/c skin graft injection model was established, the models were injected with C57/BALB/c mixed splenocytes suspension and third party DBA/BALB/c mixed splenocytes suspension respectively. The proportion of mixed cell suspension and killing rate of donor cells were detected after cells injection. The homologous series skin transplantation control group was setted, and compared with immunocompromised nude mice control group and immunosuppressant control group.   
RESULTS AND CONCLUSION: The C57/BALB/c suspension in model group and C57/BALB/c in immunosuppressant group had a strong specific cytotoxicity on C57 splenocytes of the donors. The specific cytotoxicity of C57/BALB/c suspension in immunosuppressant group was weak, and there was no obvious specific cytotoxicity in the cells at 2-4 hours after third party DBA/BALB/c mixed splenocytes injection. However, the cells of immunocompromised nude mice had no specific cytotoxicity. The antigen-specific immune status detection method established in the experiment can detect the immune status of skin transplant recipients rapidly.

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