Histological changes of oligodendrocyte precursor cells for repair of spinal cord injury

doi:10.3969/j.issn.2095-4344.2012.49.013

Abstract

Abstract:

BACKGROUND: The incidence of spinal cord injury shows an increasing tendency, but the repair mechanism after spinal cord injury is not fully understood.
OBJECTIVE: To investigate the effect of oligodendrocyte precursor cells in repair of spinal cord injury.
METHODS: According to Allen's method, models of spinal cord injury were established in mice. The morphological change of spinal cord was detected by pathological method. Oligodendrocyte precursor cells were isolated and purified from green fluorescence protein transgenic mice in vitro, and induced to differentiate into oligodendrocytes. Furthermore, oligodendrocyte precursor cells were transplanted into mice model of spinal cord injury. The experiment was divided into four groups according to different treatment methods: model group, sham-operation group, treatment group and control group.
RESULTS AND CONCLUSION: The success rate for establishing the mice model of spinal cord injury was 100%. The cultured oligodendrocyte precursor cells had the ability to self-proliferate and differentiate into oligodendrocytes. After being transplanted, oligodendrocyte precursor cells could not only integrate with the host tissue of spinal cord, but also could migrate to the injury zone and replace the damaged tissue. Motor function of mice was significantly recovered by oligodendrocyte precursor cells transplantation. Exogenous oligodendrocyte precursor cells can survive in the injury zone and integrate with the host tissue of the mice after spinal cord injury.

CLC Number:

R394.2

Kong Jian, Zhang Hong-nan, Jia Dan, Wang Duo, Chen Ming-wei, Xue Meng-meng, Li Yan, Fan Ye-wen, Liu Xin, Liu Sheng-liang, Liu Jing-jing, Ge Xiao-ping, Jiang Zhe, Wu Shu-liang. Histological changes of oligodendrocyte precursor cells for repair of spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9192-9195.

Figures/Tables 3

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