Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (49): 9157-9161.doi: 10.3969/j.issn.2095-4344.2012.49.007

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Platelet-rich fibrin induces the differentiation of bone marrow mesenchymal stem cells into
Schwann cells

Feng Yu-hua1, Dong Jing1, Lu Lei2, Li Qi3, Song Lei4   

  1. 1Affiliated Hosital of Jilin Medical College, Jinlin 132013, Jilin Province, China; 2Department of Neurology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China; 3Implantation Centre, Stomatology Hospital, Jilin University, Changchun 130021, Jilin Province, China; 4Department of Neurology, First Hospital of Norman Bethune College of Medicine of Jilin University, Changchun 130021, Jilin Province, China 
  • Received:2012-04-10 Revised:2012-06-21 Online:2012-12-02 Published:2013-01-16
  • About author:Feng Yu-hua, Attending physician, Affiliated Hosital of Jilin Medical College, Jinlin 132013, Jilin Province, China articlehelp@163.com

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Abstract:

BACKGROUND: Platelet-rich fibrin is known as a novel platelet condensate. Several studies have demonstrated that platelet-rich fibrin can induce the differentiation of bone marrow mesenchymal stem cells into neuronal-like cells.
OBJECTIVE: To investigate the effects of platelet-rich fibrin on differentiation of bone marrow mesenchymal stem cells into Schwann cells.
METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and randomized to three groups. In the control group, conventional antioxidants were used. In the platelet-rich fibrin 1 group, one piece of platelet-rich fibrin was added to induce cell differentiation. In the platelet-rich fibrin 2 group, two pieces of platelet-rich fibrin were added to induce cell differentiation.
RESULTS AND CONCLUSION: At days 3, 7, 14, 21 days of culture, CCK-8 assay was performed to detect cell toxicity. Results showed that cell proliferation at each time point was faster in the platelet-rich fibrin 1 group than that in the control group (P < 0.05), and it was faster in the platelet-rich fibrin 2 group than that in the platelet-rich fibrin 1 group (P < 0.05). Real-time PCR results showed that at day 21 of culture, intracellular S-100 and glial fibrillary acidic protein mRNA expression levels were significantly higher in the platelet-rich fibrin 1 group than those that in the control group (P < 0.05), and no significant differences were observed between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05). Flow cytometry results showed that at day 21 of culture, the proportions of intracellular S-100 and glial fibrillary acidic protein positive cells in the platelet-rich fibrin 1 group were significantly higher than those in the control group (P > 0.05) and there was no significant difference between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05).  Results indicate that platelet-rich fibrin can promote the proliferation of bone marrow mesenchymal stem cells in a dose-dependent manner, and platelet-rich fibrin can induce bone marrow mesenchymal stem cells to differentiate into a high proportion of Schwann cells. The platelet-rich fibrin induction is superior to conventional chemical inducation.

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