Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3520-3524.doi: 10.3969/j.issn.1673-8225.2012.19.021

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Feasibility of chlormethyl-benzamidodialkylcarbocyanine labeling rat kidney fat capsule adipose-derived mesenchymal stem cells 

Zi Li-ying1, Zheng Hong-guang2, Zhang De-wei2, Mei Yu-ming2   

  1. 1Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Jinzhou 121011, Liaoning Province, China; 2the General Hospital of Shenyang Military Region, Shenyang  110016, Liaoning Province, China
  • Received:2012-01-28 Revised:2012-02-24 Online:2012-05-06 Published:2012-05-06
  • Contact: Zheng Hong-guang, Chief physician, Professor, Master’s supervisor, the General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
  • About author:Zi Li-ying★, Studying for master’s degree, Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Jinzhou 121011, Liaoning Province, China ziliyingyinglizi@163.com
  • Supported by:

    Key Technologies Research and Development Program of Liaoning Province in 2011*

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Abstract:

BACKGROUND: Chlormethyl-benzamidodialkylcarbocyanine (CM-DiI) has been considered a relatively stable lipophilic fluorescent dye.
OBJECTIVE: To investigate the labeling effects of CM-Dil for kidney fat capsule adipose-derived mesenchymal stem cells and its effects on cell morphology, growth and proliferation.
METHODS: Sprague-Dawley rat kidney fat capsule adipose-derived mesenchymal stem cells were isolated by collagenase digestion. Passage 3 kidney fat capsule adipose-derived mesenchymal stem cells were labeled by CM-Dil.
RESULTS AND RESULTS: Under fluorescent microscope, CM-Dil-labeled kidney fat capsule adipose-derived mesenchymal stem cells exhibited red fluorescence, with a labeling rate of over 95%. There were no obvious changes in cell morphology, growth and proliferation before and after labeling. After several passages, the number and staining intensity of cells with red fluorescence were decreased. At 15 days after CM-Dil labeling, over 50% of passaged cells were labeled. These suggest that CM-Dil can effectively label fat capsule adipose-derived mesenchymal stem cells and this method is easy-to-operate, with a high labeling rate, produces no cytotoxicitry and does not influence cell biological characteristics. 

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