Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3511-3514.doi: 10.3969/j.issn.1673-8225.2012.19.019

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Preventive and therapeutic effects of intra-bone marrow cavity injection of bone mesenchymal stem cells on osteoporosis in rats subjected to simulated weightlessness

Wu Li-feng1, Sun Ping2, Mai Yan-xing1, Liu Hai-ming1, Xu Yan-hua1, Yang Rui1, Huang Zhen3   

  1. 1Department of Endocrinology,  3Department of Surgery, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China; 2Department of Osteoporosis, the First Affiliated Hospital of Guangdong Pharmaceutical College, Guangzhou  510080, Guangdong Province, China
  • Received:2011-09-15 Revised:2011-10-03 Online:2012-05-06 Published:2012-05-06
  • Contact: Huang Zhen, Professor, Master’s supervisor, Department of Surgery, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China hzhen2@sina.com
  • About author:Wu Li-feng★, Studying for master’s degree, Department of Endocrinology, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China 401880198@qq.com

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Abstract:

BACKGROUND: Simulated weightlessness can inhibit the proliferation of bone marrow mesenchymal stem cells and their differentiation towards osteoblasts, which leads to osteoporosis characterized by low bone mass and microarchitectural deterioration of bone tissue.
OBJECTIVE: To study the effects of intra-bone marrow cavity injection of allogeneic bone mesenchymal stem cells (BMSCs) on the bone mineral density and microarchitecture of the tibias in rats subjected to simulated weightlessness.
METHODS: Male SD rats were randomly divided into three groups: normal control group, tail-suspended simulated weightlessness group, and treatment group. The bilateral tibias were subjected to intra-bone marrow cavity injection of allogeneic BMSCs while giving the tail-suspended simulated weightlessness.
RESULTS AND CONCLUSION: Compared with the normal control group, the bone mineral density, percentage of trabecular area, trabecular number and trabecular thickness were decreased in the tail-suspended simulated weightlessness group (P < 0.01), while the trabecular separation was increased significantly (P < 0.01). Compared with the tail-suspended simulated weightlessness group, the bone mineral density, percentage of trabecular area, trabecular number and trabecular thickness were increased in the treatment group (P < 0.01), while the trabecular separation was decreased obviously (P < 0.01). The intra-bone marrow cavity injection can increase the bone mineral density of rats subjected to simulated weightlessness, improve the bone ultrastructure, slow down bone loss, and have beneficial preventive and therapeutic effects on osteoporosis..
 

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