Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3487-3491.doi: 10.3969/j.issn.1673-8225.2012.19.014

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Induced differentiation of umbilical cord blood mesenchymal stem cells into urothelial cells on fibrin clots

Guang Qian-qian1, Han Yu-huan2, Chang Ji-wu3   

  1. 1Tianjin Institute of Urinary Surgery, the State Key Laboratory of China, Tianjin Medical University, Tianjin  300070, China; 2Second Hospital of Tianjin Medical Unversity, Tianjin  300211, China; 3Tianjin Institute of Urinary Surgery, Tianjin  300211, China
  • Received:2012-01-18 Revised:2012-02-26 Online:2012-05-06 Published:2012-05-06
  • Contact: Chang Ji-wu, Professor, Tianjin Institute of Urinary Surgery, Tianjin 300211, China changjiwu2004@ yahoo.com.cn
  • About author:Guang Qian-qian★, Master, Tianjin Institute of Urinary Surgery, the State Key Laboratory of China, Tianjin Medical University, Tianjin 300070, China Guangqianqian1986@163.com

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Abstract:

BACKGROUND: Whether umbilical cord blood mesenchymal stem cells (UCB-MSCs) can be induced to differentiate into urothelial cells on fibrin clots remains poorly understood.
OBJECTIVE: To investigate the possibility of UCB-MSCs differentiating into urothelial cells on fibrin clots.
METHODS: In the experimental group, the UCB-MSCs on umbilical cord blood fibrin clot were co-cultured with fetal urothelial cells. In the control group, the UCB-MSCs on umbilical cord blood fibrin clot were cultured in culture medium of DMEM/F12.
RESULTS AND CONCLUSION: In the experimental group, the UCB-MSCs gradually became larger and were polygonal. The cells turned over their ultrastructure to urothelial cells under transmission electron microscope and were positive for immunhistochemistry staining of anti-cytokeratins (AE1/AE3). In the control group, the UCB-MSCs morphology presented spindle-like arrangement and were negative for immunohistochemistry staining of anti-cytokeratins (AE1/AE3). The UCB-MSCs on fibrin clot have the potential to differentiate into urothelial cells.

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