Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3482-3486.doi: 10.3969/j.issn.1673-8225.2012.19.013

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Protective effects of human umbilical cord mesenchymal stem cells on lung fibrosis in newborn rats 

Tu Hui-ying1, Wu Ben-qing1, Chen Li1, Zhang Yi2, Chen Zi-sheng3   

  1. 1Department of Newborn; 2Clinical Research Center; 3Department of Respiratory Medicine, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen  518020,Guangdong Province, China
  • Received:2011-07-22 Revised:2011-10-16 Online:2012-05-06 Published:2012-05-06
  • Contact: Wu Ben-qing, Master, Chief physician, Professor, Department of Newborn, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China wubenqing783@126.com
  • About author:Tu Hui-ying★, Studying for master’s degree, Department of Newborn, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China 267241372@qq. com
  • Supported by:

    the Key Special Medical Science of Shenzhen City in 2009, No. 20091998A038*; the Science and Technology Project Foundation of Shenzhen City in 2010, No. 201002001*

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Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUMSCs) have become the hot spot of cytotherapy research about chronic lung disease, because of its capacity of self-prolifetion, differentiation and paracrine effect.
OBJECTIVE: To study the protective effects of hUMSCs on lung fibrosis and normal lung growth in newborn rats.
METHODS: Thirty-two 2-day-old newborn rats were randomly divided into four groups: PBS control group, hMSCs group, bleomycin group and bleomycin+hUMSCs group. Rats in the latter two groups were prepared into models of lung fibrosis by
intraperitoneal injection of bleomycin, and two cell groups were injected with huMSCs at 7 days.
RESULTS AND CONCLUSION: In the bleomycin group, the hydroxyproline content was the highest, and pulmonary fibrosis was the most severe compared with other three groups (P < 0.05). Compared with PBS control and hUMSCs groups, the radial alveolar count and transforming growth factor β1 expression in lung tissue homogenate were significantly lower, but the mRNA expression of vascular endothelial growth factor was significantly increased in the bleomycin and bleomycin+hUMSCs groups (both P < 0.05). After treatment, the above indices were significantly reversed (P < 0.05). It indicates that hUMSCs can increase the mRNA expression of vascular endothelial growth factor, reduce the level of transforming growth factor β1 mRNA and can produce a protective effect on bleomycin induced fibrosis. However, there is no obvious difference on hydroxyproline content after hUMSCs intraperitoneal injection, it confirms that hUMSCs have no effect on normal lung development. 

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