Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3467-3470.doi: 10.3969/j.issn.1673-8225.2012.19.010

Previous Articles     Next Articles

Role of CD4+ and interferon-γ-inducible nitric oxide synthane-nitric oxide pathway in treatment of myasthenia gravis by transplantation of umbilical cord mesenchymal stem cells

Liu Hong-yan, Guo Jing-ming, Wang Hai-yan, Ye Song, Ran Chang-li   

  1. First Clinical Medical College of China Three Gorges University & Department of Hematology of Yichang Central People’s Hospital & Oncology Institute of China Three Gorges University, Yichang  443003, Hubei Province, China
  • Received:2012-02-02 Revised:2012-04-19 Online:2012-05-06 Published:2012-05-06
  • Contact: Guo Jing-ming, Master, Chief physician, First Clinical Medical College of China Three Gorges University & Department of Hematology of Yichang Central People’s Hospital & Oncology Institute of China Three Gorges University, Yichang 443003, Hubei Province, China 643025@qq.com
  • About author:Liu Hong-yan★, Master, Associate chief physician, First Clinical Medical College of China Three Gorges University & Department of Hematology of Yichang Central People’s Hospital & Oncology Institute of China Three Gorges University, Yichang 443003, Hubei Province, China liuhy518@163.com

PDF

312

Abstract:

BACKGROUND: Several studies have demonstrated that CD4+ and interferon-γ (INF-γ)-inducible nitric oxide synthane (iNOS)-nitric oxide (NO) pathway is closely related to occurrence of myasthenia gravis.
OBJECTIVE: To investigate the mechanism underlying CD4+ and IFN-γ-iNOS-NO pathway in treatment of myasthenia gravis by transplantation of umbilical cord mesenchymal stem cells.
METHODS: Rat models of myasthenia gravis were treated by intravenous transplantation of umbilical cord mesenchymal stem cells. At the same time, a control group was set. The expression of CD4+ on lymphocytes was tested by flow cytometry, the level of IFN-γ, iNOS and NO by ELISA, colorimetry and Griess, respectively.
RESULTS AND CONCLUSION: At 1 week after transplantation, the expression of CD4+ in the transplantation group was significantly higher (P < 0.01), while the level of IFN-γ, iNOS and NO was significantly lower, than in the model group (P < 0.01). These findings suggest that transplantation of umbilical cord mesenchymal stem cells can up-regulate the expression of CD4+ in the lymphocytes in the transplantation group to adjust IFN-γ-iNOS-NO pathway, down-regulate NO level so as to alleviate immune injuries.

CLC Number: