Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3447-3452.doi: 10.3969/j.issn.1673-8225.2012.19.006

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Effects of vascular endothelial cells on runt-related transcription factor 2 expression and osteogenic differentiation of bone marrow mesenchymal stem cells in a co-cultivation system

Yang Rui-nian1, Liu Liu1, Wang Fu-ke1, Zhao De-ping2   

  1. 1Department of Head and Neck Surgery, 2Experimental Center, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Received:2012-01-23 Revised:2012-02-23 Online:2012-05-06 Published:2012-05-06
  • Contact: Liu Liu, Professor, Doctoral supervisor, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China liuliu3939@126.com
  • About author:Yang Rui-nian☆, Studying for doctorate, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China 13888425351@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960388*; 81160239*

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Abstract:

BACKGROUND: Vascular endothelial cells can promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), which provide nutrition supports for osteogenic differentiation of stem cells.
OBJECTIVE: To investigate the effects of human umbilical vein endothelial cells (HUVECs) on the morphology, growth, differentiation of BMSCs as well as the runt-related transcription factor 2 (Runx2) gene expression in a co-cultivation system.
METHODS: In a co-cultivation system, passage 3 BMSCs and passage 3 HUVECs were cultured together and served as experimental group. Passage 3 BMSCs were simply cultured and served as control group. At 4, 6, 10 days of culture, BMSCs morphology, alkaline phosphatase activity and Runx2 gene expression were determined. 
RESULTS AND CONCLUSION: Compared with control group, BMSCs morphology was diverse, and cell conjunction and fusion appeared, and osteogenic differentiation was obvious during anaphase in the experimental group. At each time point, there was no obvious change in alkaline phosphatase activity in the control group, and alkaline phosphatase activity was significantly higher in the experimental group than in the control group (P < 0.05). At 6 and 8 days, Runx2 gene expression in the experimental group was about 4 times that in the control group (P < 0.01). This suggests that in the co-cultivation system, intravenous endothelial cells can promote Runx2 expression in BMSCs and induce osteogenic differentiation of BMSCs.

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