Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2889-2892.doi: 10.3969/j.issn.1673-8225.2012.16.009

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Biocompatibility of new nano-structured biomimetic bone matrix co-cultured with goat adipose derived stromal cells  

Guo Hong-gang1, Liu Jing2, Li Feng-tan1, Yang Shao-guang3, Chen Zhi4, Yao Fang-lian5, Dai Feng-ying6, Liu Wen-guang6   

  1. 1Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin  300052, China; 2Central Obstetrics and Gynecology Hospital of Tianjin, Tianjin  300025, China; 3Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin  300052, China; 4School of Precision Instrument and Opto-electronics Engineering, 5School of Chemical Engineering and Technology,  6School of Materials Science and Engineering, Tianjin University, Tianjin  300072, China
  • Received:2011-09-06 Revised:2011-10-29 Online:2012-04-15 Published:2012-04-15
  • About author:Guo Hong-gang☆, Doctor, Associate professor, Master’s supervisor, Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Supported by:

    National Natural Science Foundation of China, No. 81041061*; Technology Foundation of Tianjin Education Commission, No. 20090125*

Abstract:

BACKGROUND: Development of a bone tissue scaffold using biomimetic strategy has become the key link in development of biological substitutes currently. Imitation of natural bone composition and structure is important in development of bone matrix using biomimetic strategy.
OBJECTIVE: To analyze the biocompatibility of nano-scaled β-tricalcium phosphate/chitosan/polycaprolactone (nβ-TCP-CS-PCL) and to evaluate its biological safety.
METHODS: Nanostructured bionic bone matrix was developed based on three-dimensional image reconstruction. Goat adipose derived stromal cells were isolated and co-cultured for osteogenic induction. The experimental group: nβ-TCP-CS-PCL+gel+ recombinant human bone morphogenetic protein-2+adipose derived stromal cells. The control group: gel+recombinant human bone morphogenetic protein-2+adipose derived stromal cells.
RESULTS AND CONCLUSION: Cells grew well with abundant extracellular matrix in the experimental group. The cell proliferation activity, alkaline phosphatase level and osteocalcin level were increased in the experimental group compared with the control group (P < 0.05). New bone was gradually mature and there was no inflammatory response in the experimental group. The nβ-TCP-CS-PCL can be used as an ideal scaffold of bone tissue engineering for its good biocompatibility.

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