Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2823-2826.doi: 10.3969/j.issn.1673-8225.2012.15.036

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Hindlimb suspension affects the expression and distribution of Dystrophin in rat soleus and serum lactate dehydrogenase activity  

Ma Le-ping1, Dai Zhong-quan2, Wu Feng2, Wang Hong-hui2, Cao Hong-qing2, Wan Yu-min2, Liu Hong-yu1, Li Ying-hui2   

  1. 1Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan  030051, Shanxi Province, China; 2China Astronaut Research & Training Center, State Key Laboratory of Space Medicine Fundamentals and Application, Beijing  100094, China
  • Received:2012-02-09 Revised:2012-02-27 Online:2012-04-08 Published:2012-04-08
  • Contact: Corresponding author: Dai Zhong-quan, Doctor, Associate researcher, China Astronaut Research & Training Center, State Key Laboratory of Space Medicine Fundamentals and Application, Beijing 100094, China daizhq77@163.com Corresponding author: Liu Hong-yu, Doctor, Professor, Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan 030051, Shanxi Province, China lhyxian@nuc.edu.cn
  • About author:Ma Le-ping★, Studying for master’s degree, Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan 030051, Shanxi Province, China leping2005@126.com
  • Supported by:

    the Major State Basic Research Development Program of China (973 Program), No. 2011CB707704*; the National Natural Science Foundation of China, No. 30870589*; the Project of the State Key Laboratory of Space Medicine Fundamentals and Application, No. SMFA09A08*

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Abstract:

BACKGROUND: Dystrophin is the most prone to change in sports injuries. 
OBJECTIVE: To investigate the effects of simulated weightlessness on the Dystrophin expression and distribution in the soleus muscle, as well as the activity changes of lactate dehydrogenase in serum.
METHODS: A rat hindlimb suspension model was used to simulate weightlessness effect. After 1, 4, 7, 10 and 14 days of hindlimb suspension, the soleus and serum were isolated from SD rats for parameter examination.
RESULTS AND CONCLUSION: With the prolonged hindlimb suspension, muscle fiber cross-sectional area decreased and immunofluorescence results showed that Dystrophin expression exhibited a diffuse distribution trend, even appeared with disrupted distribution on the sarcolemma. Quantitative PCR results showed that the hindlimb suspension resulted in reduced expression of Dystrophin mRNA. Lactate dehydrogenase activity during 7-day hindlimb suspension appeared with a significant increase. Muscle atrophy induced by hindlimb suspension is accompanied with diffusion of Dystrophin distribution and decreased expression of Dystrophin mRNA. Activity changes of lactate dehydrogenase indicate that muscular atrophy may be associated with muscle injury.

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