Abstract:

BACKGROUND: The oral environments of diabetic patients were special, and there are few studies on safety of dental restorative materials for these patients.
OBJECTIVE: To observe the effects of three kinds of noble ceramic alloys and high glucose on proliferation of mouse fibroblasts L-929 cultured in vitro.
METHODS: 89% Au-base alloy, 74% Au-base alloy and silver-palladium alloy were immersed in mediums with different glucose concentrations (11.1, 22.2, and 33.3 mmol/L) for extraction. Mouse fibroblasts L-929 were co-cultured with the extraction. The control group was set up.
RESULTS AND CONCLUSION: Proliferative activities of L-929 cells cultured in mediums with different glucose concentrations and different noble ceramic alloys were different, and the two-factor interactions at 24 and 72 hours existed (P < 0.05). The proliferation of L-929 cells cultured by 89% Au-base alloy and 22.2 mmol/L high glucose concentration was more faster than the control group (P=0.004), while the silver-palladium alloy could inhibit the proliferation (P < 0.001). However, no significant difference was found among the 74% Au-base alloy groups with different glucose concentrations and the control group. It is suggested that 74%Au-alloy with different glucose concentrations among these three kinds of materials has the least effects on cell proliferation.