Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (12): 2158-2162.doi: 10.3969/j.issn.1673-8225.2012.12.016

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Correlation between central venous catheter-related staphylococcus epidermidis icaA, icaD, transforming growth factor beta 1 and biofilms in lung cancer patients**☆

Lei Yu-jie1, Huang Yun-chao1, Yang Li2, Guo Feng-li2, Xu Yu-shan3, Ye Lian-hua1, Chen Xiao-bo1,  Zhao Guang-qiang1, He Meng1   

  1. 1Department of Cardiothoracic Surgery, 2Department of Clinical Laboratory, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming  650118, Yunnan Province, China; 3Department of Endocrinology, First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-07-13 Revised:2011-08-15 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Huang Yun-chao, Professor, Doctoral supervisor, Department of Cardiothoracic Surgery, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming 650118, Yunnan Province, China huangych2001@ yahoo.com.cn
  • About author:Lei Yu-jie☆, Studying for doctorate, Lecturer, Department of Cardiothoracic Surgery, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming 650118, Yunnan Province, China leiyujie952499@ yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30872555*; the Application Basis of Science and Technology Development Plan of Yunnan Province, No. 2009ZC116M*

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Abstract:

BACKGROUND: It is confirmed that pathogenicity of biomaterials-centered infection is positive correlated to bacterial biofilm formation of Staphylococcus epidermidis on the surface of catheter-related materials. 
OBJECTIVE: To analyze the relations between expressions of central venous catheter-related Staphylococcus epidermidis icaA, icaD, transforming growth factor β1 (TGF-β1) and formation of bacterial biofilm.
METHODS: The type of Staphylococcus epidermidis in lung cancer cases with catheter-related bloodstream infections (CRBSI) was indentified, followed by bacterial genomic DNA extraction. The expression of biofilm formation-related genes icaA, icaD mRNA and phenotype of biofilm were detected by PCR. The serum TGF-β1 levels in cases with or without CRBSI were measured by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The expression of Staphylococcus epidermidis operon icaA and icaD gene was positive correlated to biofilm formation in lung cancer cases with CRBSI (P < 0.01); particularly, the TGF-β1 levels in CRBSI cases were greater than that of non-CRBSI cases (P < 0.05). The results demonstrated that, central venous catheter infection causes positive Staphylococcus epidermidis icaA and icaD gene expressions in lung cancer cases and is prone to form biofilm, high level of peripheral TGF-β1 may play a positive role in bacterial biofilm formation.

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