Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (11): 1985-1988.doi: 10.3969/j.issn.1673-8225.2012.11.020

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Expression of interleukin-17 in the myocardium of rats with myocardial infarction

Chen Zhao-zhe1, 2, Chen Zhi-jian1, Zeng Qiu-tang1, Liu Xue-gang1, Zhang Qing1, Liu Li-xin1, Zhu Yun-tao1, Qian Cheng1, Du Yi-mei1, Zhao Ning1   

  1. 1Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China; 2Department of Cardiology, Central Hospital of Xinyang, Xinyang  464000, Henan Province, China
  • Received:2011-09-06 Revised:2011-12-03 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Chen Zhi-jian, Doctor, Chief physician, Professor, Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China drchenok@sohu. com
  • About author:Chen Zhao-zhe☆, Studying for doctorate, Associate chief physician, Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Cardiology, Central Hospital of Xinyang, Xinyang 464000, Henan Province, China sunfly1020@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30770880*, 3097124*

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Abstract:

BACKGROUND: Expression of interleukin-17 increases in many cardiovascular disease such as autoimmune myocarditis, viral myocarditis, dilated cardiomyopathy and unstable coronary artery disease. Interleukin-17 plays an important role in chronic inflammatory process of cardiovascular diseases.
OBJECTIVE: To study the expression of interleukin-17 in the myocardium after myocardial infarction in rats.
METHODS: Liquid nitrogen freezing method was used to establish a rat myocardial infarction model. Totally 66 rats with modeling successful were randomly divided into six groups according to different observation times: 1, 3, 7, 14, 28, 56 days groups. And eight rats were selected as controls. The expression of interleukin-17 was examined by enzyme linked immunosorbent assay, laser confocal scanning microscope and Western blot.
RESULTS AND CONCLUSION: Modeling success rate was 73%. With the development of myocardial infarction, interleukin-17 was abundantly expressed in the myocardial tissue and gradually increased over time. The expression of interleukin-17 in the 28 days group increased most significantly. Interleukin-17 as a potent pro-inflammatory factor can strongly express in rat myocardium after myocardial infarction, which may be related to the incidence of ventricular remodeling and arrhythmia.

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