Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (11): 1989-1993.doi: 10.3969/j.issn.1673-8225.2012.11.021

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Construction of a rabbit ear model of chronic wound using transfixion ligation combined punch method

Li Li1, Tong Ya-lin2, Zhan Qiu2, Lin Yuan1   

  1. 1Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2Department of Burns and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin  541002, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-25 Revised:2011-10-25 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Lin Yuan, Chief physician, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gx-linyuan8@163.com
  • About author:Li Li★, Studying for master’s degree, Physician, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China chenfeng5891429@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi, No. 0728255*

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Abstract:

BACKGROUND: In the past, the constructed rabbit ear models of chronic wound are limited to simple models of ischemia, while the model of both ischemia and congestion has been rarely reported.
OBJECTIVE: To establish a chronic wound model for ischemia and congestion using rabbit ears.
METHODS: A total of 40 rabbit ears were divided into two groups, experiment group and control group. The area of the dorsoventral side in ear root, all cephalic and center arteries and veins were transfixion ligated in the experiment group. Three circular wounds as deep as the cartilage surface were punched at the dorsal sides of the ears, using biopsy punch on the 10th day after ligation. Three circular wounds identical to that of the experiment group were punched in the control group.
RESULTS AND CONCLUSION: The color of rabbit ears in the experiment group was deep violet-black immediately after ligation, and was somewhat violet-black, violet and lilac on the 10th, 20th and 30th days after ligation. It illustrates that the status of ischemia and congestion is durable and stable in the chronic wound model constructed using transfixion ligation combined punch method. Compared with the control group, the time of granulation tissue growth, the time of wound healing and epidermidalization in the experiment group were obviously delayed (P < 0.01). The expression of vascular endothelial growth factor in the experiment group was obviously lower than that of the control group (P < 0.01).These findings demonstrate that rabbit ear models of chronic wound constructed using transfixion ligation combined punch method are simple and reliable. It can be used as an ideal animal study model in the research of chronic wounds repair caused by ischemia and congestion factors.

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