Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1795-1798.doi: 10.3969/j.issn.1673-8225.2012.10.019

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Effect of uric acid on proliferation of human placenta-derived mesenchymal stem cells

Cui Jing1, Yang Nai-long1, Wang Jing-yi2   

  1. 1Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao Medical University, Qingdao  266003, Shangdong Province, China; 2Department of Obstetrics, Queen Mary Hospital of Qingdao, Qingdao  266003, Shangdong Province, China
  • Received:2011-07-30 Revised:2011-09-26 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Yang Nai-long, Master, Chief physician, Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, Shangdong Province, China nailongy@163.com
  • About author:Cui Jing★, Studying for master’s degree, Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, Shangdong Province, China cuijing512@163.com

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Abstract:

BACKGROUND: Uric acid (UA) plays different roles on different cells. But, what is the effect of UA on the proliferation of human placenta-derived mesenchymal stem cells (hPMSCs), promoting or suppression?
0BJECTIVES: To observe the effect of UA on proliferation of hPMSCs, and to explore the relationship between time-effect and dose-effect.
METHODS: The hPMSCs cultured in vitro were used and divided into 6 groups: the control group and the UA group which contained 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA.
RESULTS AND CONCLUSION: MTT assay showed that the number of hPMSCs in 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA group was more than that in the control group at the same time, especially in 0.8 mmol/L UA group; the number of hPMSCs in same concentration of UA was increasing following the time (time﹤5 d), and reached to peak at the 5th day. UA could accelerate the proliferation of hPMSCs and showed a time- and dose-dependent manner, and the effect of 0.8 mmol/L UA on the proliferation capacity of hPMSCs was stronger than others at the 5th day.

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