Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1761-1764.doi: 10.3969/j.issn.1673-8225.2012.10.012

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Effect of recombinant human erythropoietin on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women

Liu Yan1, Li Fa-Qi1, Zhang Bin2, Luo Jian-Ping3   

  1. 1Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2Department of Cardiology, the General Hospital of Chongqing Iron and Steel Group, Chongqing 400080, China; 3Center of Prenatal Diagnosis, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
     
  • Received:2012-01-09 Revised:2012-02-02 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Li Fa-Qi, M.D., Professor, Chief physician, Master’s supervisor, Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China faqili_2006@yahoo.com
  • About author:Liu Yan★, Studying for master’s degree, Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China liuyan2009110177@163.com
  • Supported by:

    Medical Scientific Foundation of Chongqing Health Bureau, No. 2009-2-367*

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Abstract:

BACKGROUND: Studies have shown that amniotic fluid stem cells of pregnancy women can differentiate into myocardial precursor cells.
OBJECTIVE: To study the effect of recombinant human erythropoietin (rhEPO) at different concentrations on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women.
METHODS: The differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women was induced by adding conditional culture medium containing 0, 1.0, 5.0, 10.0, 20.0 U/mL rhEPO. After 24 hours, conditional culture medium was replaced by complete medium.
RESULTS AND CONCLUSION: During 14 days of induced differentiation, under the inverted microscope, cells gradually shortened and widened from original shuttle appearance. Adjacent cells fused together, similar to myotube-like structure. The differentiation rate of myocardial precursor cells was highest after 5.0 U/mL rhEPO addition. RT-PCR results showed that rhEPO particularly at the concentration of 5.0 U/mL could promote Nkx-2.5 and GATA-4 mRNA expression. These findings suggest that exogenous rhEPO can promote the differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women dose-dependently.

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