Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1756-1760.doi: 10.3969/j.issn.1673-8225.2012.10.011

Previous Articles     Next Articles

Simulation of stem cell microenvironment in vitro to culture and expand cord and umbilical cord mesenchymal stem cells

Qiu Yun, Zheng Qing, Xiao Shu-dong, Wang Zheng   

  1. Department of Digestion Medicine, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai Institute of Digestive Disease, MED-X Renji Stem Cell Clinical Research Center, Shanghai  200001, China
  • Received:2011-09-10 Revised:2011-12-25 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Zheng Qing, M.D., Associate professor, Department of Digestion Medicine, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai Institute of Digestive Disease, MED-X Renji Stem Cell Clinical Research Center, Shanghai 200001, China qingzheng101@yahoo.com arthor: Wang Zheng, M.D., Associate professor, Department of Digestion Medicine, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai Institute of Digestive Disease, MED-X Renji Stem Cell Clinical Research Center, Shanghai 200001, China zheng.w.dr@gmail.com
  • About author:Qiu Yun★, Studying for master’s degree, Department of Digestion Medicine, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai Institute of Digestive Disease, MED-X Renji Stem Cell Clinical Research Center, Shanghai 200001, China
  • Supported by:

    Pujiang Talent Plan Program of Science Committee of Shanghai, No. 09PJ1407300*; the National Natural Science Foundation of China, No. 30971468*

PDF

415

Abstract:

BACKGROUND: The future of cell therapy requires high purity of mesenchymal stem cells (MSCs) in order to clearly determine the therapeutic dose and dose-response relationship. Establishing a robust amplification system in vitro to obtain the therapeutic dose of stem cells while preserving the characteristics of stem cells is the urgent of clinical laboratories.
OBJECTIVE: To simulate a microenvironment for stem cells in vitro to harvest and expand umbilical cord blood-derived and cord-derived MSCs and to compare with conventional two-dimensional culture system.
METHODS: The umbilical cord blood-derived and cord-derived MSCs were inoculated in the conventional two-dimensional plastic culture system and extracellular matrix, respectively. The two amplification systems for in vitro large-scale expansion of umbilical cord blood-derived and cord-derived MSCs were evaluated from the points of cell counts and surface markers.
RESULTS AND CONCLUSION: The production of umbilical cord blood-derived and cord-derived MSCs in the bone marrow-derived extracellular matrix culture system was 4-6 times of that in the conventional two-dimensional culture system. FACS analysis showed that extracellular matrix system better maintained the surface marker of stem cells. Therefore, such a three-dimensional culture system is much closer to physical environment than the two-dimensional culture system.
The established bone marrow-derived extracellular matrix culture system can maintain the characteristics of mesenchymal stem cells, which provides access to more homogeneous MSCs of high activity within a short time period.

CLC Number: