Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1737-1741.doi: 10.3969/j.issn.1673-8225.2012.10.007

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In vitro induction of human bone marrow mesenchymal stem cells to differentiate into type Ⅱalveolar epithelial cells

Chen Yin1, 2, Ma Nan2, Mei Ju2, Xiao Hai-bo2, Lu Shan-wei1, Xu Huai-Yang1, Zhong Hong1, 2   

  1. 1Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital (Chongming Branch) of Shanghai Jiaotong University, Shanghai  202150, China; 2Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiaotong University, Shanghai  202150, China
  • Received:2012-01-04 Revised:2012-01-14 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Zhong Hong, M.D., Professor, Chief physician, Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital (Chongming Branch) of Shanghai Jiaotong University, Shanghai 202150, China; Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiaotong University, Shanghai 202150, China zhonghongyyy@163.com
  • About author:Chen Yin★, Studying for master’s degree, Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital (Chongming Branch) of Shanghai Jiaotong University, Shanghai 202150, China; Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiaotong University, Shanghai 202150, China zhujichenying@126.com

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Abstract:

BACKGROUND: Alveolar epithelial type Ⅱ (AT Ⅱ) has been demonstrated to directly participate in liver recovery and can also alleviate the severity of pulmonary fibrosis in a rat model of pulmonary fibrosis. Embryonic stem cells (ESCs) can differentiate into AT Ⅱ in vitro, but the application of ESCs is confined by many factors.
OBJECTIVE: To investigate the method and transformation rate of bone marrow mesenchymalstem cells (BMSCs) differentiation into AT Ⅱ.
METHODS: The BMSCs were isolated from human bone marrow cells. BMSCs were co-cultured with human embryonic lung mesenchymal cells (MRC-5) in serum-free small airway growth medium (SAGM) and modified SAGM. The morphology of BMSCs was observed, and the expression of surfactant protein C (SPC) mRNA was detected by RT-PCR. In addition, the expression of SPC was also detected by immunofluorescence.
RESULTS AND CONCLUSION: After the BMSCs were co-cultured with MRC-5 for 10 days, some of BMSCs began to turn into epithelioid cells in morphous. After 15 days, the expression of SPC mRNA was detected, but it was not increased along with the prolongation of coculture days. Compared with SAGM, modified SAGM could significantly increase the expression of SPC mRNA (P < 0.05). BMSCs could differentiate into AT Ⅱ in SAGM or modified SAGM when co-culture with MRC-5 in vitro at low transformation rate. Positive rate of SPC was only (3.15±0.69)%.

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