Abstract:

BACKGROUND: The previous studies showed that transplantation of human amniotic epithelial cells (hAECs) could improve neurological function, but the mechanism is unclear.
OBJECTIVE: To explore the effects on neural stem cells and the expression of Neurogranin (Ng) of neonatal rats with hypoxic-ischemic encephalopathy, and the therapeutic effects of transplantation of human amniotic epithelial cells (hAECs).
METHODS: Forty-six rats were randomly divided into sham operation control, model group, transplantation group. The 1×1012/L hAECs and PBS were respectively injected into the cerebral ventricle of the transplantation group and model group, and sham operation group did not treated.
RESULTS AND CONCLUSION: Compared with model group, the neurological function was significantly improved in the transplantation group, NSE positive cells were detected in the transplantation group rat brains at 3 weeks following transplantation, Ng proteins expression in the model group was significantly lower than the sham operation group and the transplantation gorup at 3 weeks following transplantation. nestin proteins expression in the model group was higher than that in the sham operation group and lower than that in the transplantation group. Results indicated that hAECs injected into the cerebral ventricles could reduce the hypoxic-ischemic injury to neurons and improve neurological function. The mechanism was maybe achieved by autogenous neural stem cell proliferation, differentiation and synaptic regeneration stimulated by hAECs and its secretion.