Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 85-89.doi: 10.3969/j.issn.1673-8225.2012.01.018

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Effects of bone marrow mesenchymal stem cells transplantation in different ways on hippocanipal neurons following traumatic brain injury in rats

Lü Jia-xi, Yin Hong   

  1. Department of Neurosurgery, Guilin Second People's Hospital, Guilin  541001, Guangxi Province, China
  • Received:2011-07-02 Revised:2011-10-16 Online:2012-01-01 Published:2012-01-01
  • About author:Lü Jia-xi, Attending physician, Department of Neurosurgery, Guilin Second People's Hospital, Guilin 541001, Guangxi Province, China lvjiaxi005@sina.com

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Abstract:

BACKGROUND: In recent years, application of bone marrow mesenchymal stem cells (BMSCs) transplantation in the central nervous system has become more widely, and the study of the best way to transplantation is important.
OBJECTIVE: To investigate the effect of BMSCs transplantation in different ways on hippocanipal neuron following traumatic brain injury (TBI) in rats.
METHODS: BMSCs were isolated from the bone marrow of 1-month-old SD rat. The BMSCs at passage 3 were labeled with PKH26 and were observed under fluorescence microscope. The percentage of the labeled cells was detected by flow cytometer. The model of hippocampal neuronal death following TBI was established by improved Feeney’s weight drop techniques. Sixty Wistar rats were divided into three groups randomly: Injured brain area transplantation group was injected with 10 μL normal saline contained BMSCs; Vena caudalis transplantation group was injected with 1 mL normal saline contained BMSCs through vein caudalis; TBI group was not treated.
RESULTS AND CONCLUSION: Fluorescence microscope observation showed that the BMSCs labeled by PKH26 were spherical and presented with red fluorescence and uniform-distributed. Flow cytometric detection revealed that the percentage of labeled cells was over 99%. The learning and memory function of injured brain area transplantation group was significantly better than that of other two groups. The number of PKH26-positive cells and neurons after hematoxylin-eosin staining in injured brain area transplantation group was higher than that in vena caudalis transplantation group, and the number in vena caudalis transplantation group was higher that in TBI group (P < 0.05). RT-PCR approved that the expression of GAP-43 mRNA was highest in injured brain area transplantation group, a few in vena caudalis transplantation group and lowest in TBI group (P < 0.05). BMSCs transplantation plays an important role in protecting against hippocampal neuronal death following TBI. Injured brain area transplantation was significantly better than vena caudalis transplantation.

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