Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 125-129.doi: 10.3969/j.issn.1673-8225.2012.01.027

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Effect of miniature pig platelet-rich plasma on osteogenic induction of periodontal ligament stem cells in vitro

Zhang Yuan1, Zhong Liang-jun2, Zhang Peng-tao1, Zhang Yuan-ming3, Xu Yan4   

  1. 1Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China; 2Clinical Medical College of Hangzhou Normal University, Hangzhou  310015, Zhejiang Province, China; 3Heart Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 4 Affiliated Dental Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
  • Received:2011-09-23 Revised:2011-10-14 Online:2012-01-01 Published:2012-01-01
  • Contact: Zhang Yuan-ming, Professor, Doctoral supervisor, Heart Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zymdxx@163.com
  • About author:Zhang Yuan★, Studying for master’s degree, Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China actionzy@qq.com
  • Supported by:

    Project of Science and Technology Supporting to Xinjiang Uygur Autonomous Region, No. 201091144*

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Abstract:

BACKGROUND: Tissue engineering technology provides a new way for bone defects caused by periodontitis.
OBJECTIVE: To explore the effect of platelet-rich plasma (PRP) on osteogenic induction of miniature pig periodontal ligament stem cells (PDLSCs).
METHODS: Collected the Guizhou miniature pig’s venous blood and prepared the centrifuged PRP three times; tissue block method was used to culture the periodontal ligament cells. 0.8%, 1.0%, 1.2% concentration of PRP were co-cultured with PDLSCs for 3, 7, 14 and 21 days, respectively. No PRP addition as control group.
RESULTS AND CONCLUSION: Alkaline phosphatase (AKP) activity reach a peak at the 14th day after induced with 0.8%, 1.0% and 1.2% PRP, and highest activity of AKP was induced by 1.0% PRP. AKP activity was reduced at the 21st day. Alizarin red staining showed the PDLSCs were positive at 21st day after osteogenic induction of PRP. Purified monoclonal cell growth curve entering the logarithmic growth phase since the 3rd day, the amount of cells reached a peak at the 8th day. PRP can induce osteogenic PDLSC performance, especially the 1.0% PRP induction showed the optimal efficiency in vitro.
 

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