Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 103-106.doi: 10.3969/j.issn.1673-8225.2012.01.022

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In vitro directional differentiation of bone marrow mesenchymal stem cells into nerve cells induced by cistanche total glycosides

Dong Ya-li, Li Jian-feng   

  1. Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010030, Inner Mongolia Autonomous Region, China
  • Received:2011-07-23 Revised:2011-08-30 Online:2012-01-01 Published:2012-01-01
  • Contact: Li Jian-feng, Master, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China jianfeng0471@163.com
  • About author:Dong Ya-li, Chief physician, Department of Rehabilitation, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China mile711@yahoo.com.cn

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Abstract:

BACKGROUND: Cistanche and its chemical composition play a neuroprotective role, and can promote recovery of neurological function.
OBJECTIVE: To analyze the optimal dose of cistanche total glycosides for in vitro induction of bone marrow mesenchymal stem cells differentiating into nerve cells.
METHODS: Bone marrow mesenchymal stem cells of Wistar rats were isolated and then cultured. β-mercaptoethanol and different concentrations of cistanche total glycosides were used to induce bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells of Wistar rats were divided into blank control group, β-mercaptoethanol control group, cistanche total glycosides groups of 100, 200, 300 μg/L for observation.
RESULTS AND CONCLUSION: After 7 days of induction, the number of living cells increased obviously in other groups, compared with the blank control group (P < 0.05); at the 3rd day of induction, Nestin, NSE-positive expression rate was significantly higher in cistanche total glycosides groups, compared with the blank control group (P < 0.05). 100, 200 μg/L cistanche total glycosides could effectively promote proliferation of bone marrow mesenchymal stem cells, and significantly promote the differentiation into nerve cells. 300 μg/L cistanche total glycosides could promote proliferation of bone marrow mesenchymal stem cells, but the effect was less than 100, 200 μg/L (P < 0.05).

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