Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9575-9578.doi: 10.3969/j.issn.1673-8225.2011.51.018

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Biological security of nano-hydroxyapatite wisker and β-tricalcium phosphate composited bone substitute

Hu Hong-tao1, Xu Guo-hua1, Liu Ji-chun1, Jiang Yu-quan1, Tang De-zhi2, Zan Qing-feng3, Ye Xiao-jian1   

  1. 1Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai  200003, China
    2Affiliated Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai  200032, China
    3Department of Fine Ceramics, Key Laboratory of Ceramics, Institute of Nuclear and New Energy Technology of Tsinghua University, Beijing  100084, China
  • Received:2011-06-27 Revised:2011-10-16 Online:2011-12-17 Published:2011-12-17
  • Contact: Ye Xiao-jian, Professor, Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China yespine@163.com
  • About author:Hu Hong-tao☆, Studying for doctorate, Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China hht232@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872639*, 81071477*; Science and Techology Commission Foundation of Shanghai, No. 1052nm03200*

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Abstract:

BACKGROUND: The technique, which reinforces porous calcium phosphate scaffold by nano hydroxylapatite whisker in situ, can enhance the strength and toughness of scaffold.
OBJECTIVE: To evaluate the biocompatibility of the nano-hydroxyapatite wisker and β-tricalcium phosphate (nHAW/β-TCP) as tissue engineered vascular scaffold in vitro.
METHODS: The acute systemic toxicity test: Totally 30 mice were divided into intravenous injection group, intra-abdominal injection group and control group. Mice in the three groups were injected by leaching liquor and saline respectively, then the general state of the mice was recorded at the 24, 48 and 72 hours. Test of hemolysis: Dissolution of red blood cells were observed in the mixed liquids of leaching liquor and diluted blood, and the A value was detected at optical density 545 nm then the hemolytic rare was calculated. The sensitization test: Sixteen guinea pigs were randomly divided into experimental group, negative control group and postive control group. Leaching liquor from nHAW/β-TCP, normal saline and 2% dinitrofluorobenzene were injected intradermally into each side of the backbone of each guinea pig. The erythema and edema were recorded at immediate, 24, 48, 72 hours. Test of cytotoxicity: The cells were cultured in the special L-DMEM that nHAW/β-TCP were dipped in and then general morphology of cells and cell activity were observed through microscope and CCK-8.
RESULTS AND CONCLUSION: The acute systemic toxicity test: After intravenous and intra-abdominal injection of soaked of materal, there were no changes in breath, appetite, body weight of mice and no death. Test of hemolysis: The haemolytic rates of nHAW/β-TCP were less than 5% of international standard organization requirements, suggesting these materaials had no haematolysis effect. The sensitization test: No allergic reaction were found in guinea pig after endodermal injection. Test of cytotoxicity: Test of CCK-8 colormetry showed that the different densities leaching liquor had no cytotoxicity. nHAW/β-TCP does not cause acute whole body toxic reaction, haematolysis effect, allergic reaction and no cytotoxity. The materials have a good biocompatibility and meet the application requirements for tissue engineered vascular scaffold.

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