Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9570-9574.doi: 10.3969/j.issn.1673-8225.2011.51.017

Previous Articles     Next Articles

Effect of glucose on eliminating activity and adhesion of Staphylococcus epidermidis in Tupaia belangeri

Xu Yu-shan1, Wang Xi2, Xu Yun-min3, Lei Yu-jie4, He Meng4, Huang Yun-chao4   

  1. 1Department of Endocrinology and Metabolism, 3Department of Laboratory, First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China;    2Department of Cardiovascular Thoracic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming  650101, Yunnan Province, China; 4Department of Cardiovascular Thoracic Surgery, Third Affiliated Hospital of Kunming Medical College, Kunming  650118, Yunnan Province, China
  • Received:2011-05-24 Revised:2011-06-26 Online:2011-12-17 Published:2011-12-17
  • Contact: Huang Yun-chao, Professor, Department of Cardiovascular Thoracic Surgery, Third Affiliated Hospital of Kunming Medical College, Kunming 650118, Yunnan Province, China huangych2001@ yahoo.com.cn
  • About author:Xu Yu-shan☆, Doctor, Associate chief physician, Department of Endocrinology and Metabolism, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China xuyushan1@ yahoo.com.cn Wang Xi★, Master, Attending physician, Department of Cardiovascular Thoracic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China wangxi1a2b@ sina.com Xu Yu-shan and Wang Xi contributed equally to this study.
  • Supported by:

    the National Natural Science Foundation of China, No. 30872555*; Yunnan Province Science and Technology Project, No. 2007C009Z*

PDF

360

Abstract:

BACKGROUND: High-concentration glucose can induce the formation of biofilms in Staphylococcus epidermidis.
OBJECTIVE: To investigate the effect of high-concentration glucose on eliminating ability and adhesion of Staphylococcus epidermidis in Tupaia belangeri.
METHODS: Tupaia belangeri models of hyperglycaemia (≥11.1 mmoL/L) were induced by intraperitoneal injection of Streptozocin. PVC catheters were inserted into the left femoral vein, and Staphylococcus epidermidis with or without formation of biofilms was inoculated into the catheters.
RESULTS AND CONCLUSION: Following transfection of Staphylococcus epidermidis with formation of biofilms, there were higher rates of bacterial infection as well as higher bacterium colony number in the serum, heart, liver, kidney and pancreas of Tupai belangeri in hyperglycemia group (≥11.1 mmoL/L) as compared with the control group (P < 0.05). Detected by scanning electronic microscope, biofilm formation was remarkable in hyperglycemia group (≥11.1 mmoL/L) (P < 0.05). However, there was no formation of biofilms in hyperglycemia or control groups following transfection of Staphylococcus epidermidis without formation of biofilms. Hyperglycemia can induce the decreasing ability of eliminating bacteria and the increasing formation of biofilms on the surface of biomaterials transfected with Staphylococcus epidermidis.

CLC Number: