Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9445-9448.doi: 10.3969/j.issn.1673-8225.2011.50.033

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Expression of peroxidase 6 in fetal rat testicular cells in pregnant rat hypospadias model induced by di-n-butyl phthalate

Shen Bai-xin1, Wei Zhong-qing1, Ju Xiao-bing2, Zuo Wei1, He Jia1, Shi Hua-chao1, Zhang Wei2, Wang Xin-ru3   

  1. 1Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing  210011, Jiangsu Province, China
    2Department of Urinary Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
    3Nanjing Medical University School of Public Health, Nanjing 210029, Jiangsu Province, China
  • Received:2011-09-22 Revised:2011-10-21 Online:2011-12-10 Published:2011-12-10
  • Contact: Wei Zhong-qing, Professor, Master’s supervisor, Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China weizq1@163.com
  • About author:Shen Bai-xin★, Master, Department of Urinary Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China afantidoctor@163.com
  • Supported by:

    the General Project of National Natural Science Foundation of China, No. 30872596*; the General Project of Science and Technology Foundation for Nanjing Medical University, No. 08NMUM023*; the College Students' Practice Innovation Training Project for Higher Education of Jiangsu Province, No. KY112J2010002*

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Abstract:

BACKGROUND: Previous study shows that expression of peroxidase 6 in fetal rat testis increase significantly induced by in utero exposure to di-n-butyl phthalate.
OBJECTIVE: To further investigate the effect of prenatal di-n-butyl phthalate exposure on peroxidase 6 expression.
METHODS: Rats of 14 to 18 days of pregnancy were exposed to di-n-butyl phthalate with the dose of 800mg / (kg•d). Testes were harvested from the fetal rats from gestational day 21 and peroxidase 6 was extracted. The expression of peroxidase 6 was detected and analyzed by western blot and immunohistochemical methods.
RESULTS AND CONCLUSION: According to western blot, the expression of peroxidase 6 in experiment group (0.205±0.020) was significantly higher than that in the control group (0.110 ± 0.020, P < 0.05). According to immunohistochemistry, peroxidase 6 was mainly localized in Leydig cells of fetal rat testes. These findings indicate that peroxidase 6 may antagonize di-n-butyl phthalate toxicity by antioxidation and stimulating the proliferation of Leydig cells. The function of peroxidase 6 needs further research.

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