Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9449-9452.doi: 10.3969/j.issn.1673-8225.2011.50.034

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Antarctic krill trypsin promotes wound healing

Zhang Peng, Jiang Guo-liang, Wang Jun-ren   

  1. College of Marine Life Sciences, Ocean University of China, Qingdao  266003, Shandong Province, China
  • Received:2011-06-01 Revised:2011-07-21 Online:2011-12-10 Published:2011-12-10
  • Contact: Jiang Guo-liang, Professor, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong Province, China jiangguoliang@ouc.edu.cn
  • About author:Zhang Peng★, Studying for master’s degree, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong Province, China zp52715@126.com
  • Supported by:

    the Natural Science Foundation of Shandong Province, China, No. ZR2010DQ012*

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Abstract:

BACKGROUND: It is confirmed that trypsin has better effects on debridement and wound healing.
OBJECTIVE: To explore the effects of the Antarctic krill trypsin on wound healing.
METHODS: Back trauma model in New Zealand white rabbits was established by defecting full-thickness skin. There were four wounds in each rabbit, treated with crude trypsin, pure trypsin extracted from Antarctic krill, papin and saline, respectively. The wounds were treated twice daily until healed.
RESULTS AND CONCLUSION: According to the healing area at the 5th, 10th and 15th days after treatment, crude trypsin and pure trypsin extracted from Antarctic krill had a better effect on wound healing. Crude trypsin was better than pure trypsin. The residual wounds area of crude typsin group for each time period was smaller than that of saline group and papain group (P < 0.05). The content determination results of skin hydroxyproline showed that crude trypsin significantly improved the synthesis and accumulation of collagen in wound tissue. According to histological sections, crude typsin group achieved the best efficiency of the treatment. These results demonstrate that Antarctic krill trypsin can promote wound healing in New Zealand white rabbits, and the crude typsin has the best effect.

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