Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 861-864.doi: 10.3969/j.issn.1673-8225.2011.05.024

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Triptolide PG490-88 combined with ciclosporin A induces immune tolerance in a rat renal allograft rejection model

Dai Cheng, Wang Ping, Zhang Xin-tao, Huang Zhi-heng, Zhu Liang   

  1. Department of Kidney Transplantation, Shenzhen Second People’s Hospital, First Hospital of Shenzhen University, Shenzhen   518035, Guangdong Province, China
  • Received:2010-10-05 Revised:2010-12-31 Online:2011-01-29 Published:2011-01-29
  • About author:Dai Cheng★, Master, Associate chief physician, Associate professor, Department of Kidney Transplantation, Shenzhen Second People’s Hospital, First Hospital of Shenzhen University, Shenzhen 518035, Guangdong Province, China Hxming2007@yahoo.cn
  • Supported by:

    the Science and Technology Plan of Shenzhen, No. 200903038*

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Abstract:

BACKGROUND: Studies demonstrated that triptolide has good effect on anti-rejection and anti-tumor. PG490-88 is extracted from triptolide, which can prevent graft versus host disease and induce immune tolerance.
OBJECTIVE: To investigate the effect of PG490-88 combined with ciclosporin A on inducing immune tolerance in a rat renal allograft rejection model.
METHODS: Kidney transplantation models were constructed using improved method. Forty Wistar rats served as donors, and 40 SD rats served as recipients. All rats were randomly divided into 4 groups. Rats were regular raised in the control group. Rats were lavaged with triptolide PG490-88 [20 mg/(kg•d)], ciclosporin A [15 mg/(kg•d)] or PG490-88 [20 mg/(kg•d)]+ ciclosporin A
[15 mg/(kg•d)] in the PG490-88, ciclosporin A or the combination group. Serum levels of interleukin 2-receptor (IL-2R) and activity of IL-2 in spleen lymph cells were detected respectively at 1, 3, 5, 7 and 14 days after transplantation．
RESULTS AND CONCLUSION: Serum levels of IL-2R and activity of IL-2 were detected lower in 3 treated groups than those in the control group (P < 0.05). Among the 3 treated groups, the combination group was lower than that of the PG490-88 and ciclosporin A groups (P < 0.05). Triptolide PG490-88 performs its function of immunosuppressant on the liver transplantation through IL-2 and IL-2R. It is not only has immunosuppressive action, but also can induce immune tolerance, the effects would be better when combined with ciclosporin A.

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