Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9133-9136.doi: 10.3969/j.issn.1673-8225.2011.49.003

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Dose-dependent effect of erythropoietin on the proliferation of rabbit bone marrow mesenchymal stem cells

Li Zheng-zhang1, Shen Zhe1, Cheng Ying-zhang2, Xue Hao-ping1, Ji Qun1, Cheng Shi1, Xue Min-zhi1   

  1. 1Department of Cardiology, Gaoyou People’s Hospital, Gaoyou  225600, Jiangsu Province, China
    2Department of Cardiology, Second Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-05-18 Revised:2011-06-05 Online:2011-12-03 Published:2011-12-03
  • Contact: Cheng Ying-zhang, Chief physician, Associate professor, Master's supervisor, Department of Cardiology, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Li Zheng-zhang★, Master, Attending physician, Department of Cardiology, Gaoyou People’s Hospital, Gaoyou 225600, Jiangsu Province, China

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Abstract:

BACKGROUND: Some studies have certificated that erythropoietin (EPO) can promote bone marrow mesenchymal stem cells (BMSCs) proliferation, but it is not yet clear about the dose-dependent relationship.
OBJECTIVE: To explore the different-dose effects of EPO on rabbit BMSCs proliferation activity and cell cycle.
METHODS: MTT colorimetric test and flow cytometry were used to detect the effect of EPO (0, 2, 4, 8, 16 U/mL) on BMSCs cell cycle; meantime, the culture medium was collected for matrix metalloproteinase-2 (MMP-2) content detection.
RESULTS AND CONCLUSION: 2-16 U/mL EPO could promote BMSCs proliferation gradually in a dose-dependent manner. BMSCs percentage in S phase and proliferative index gradually increased with the dose increase of EPO, and the proliferation index of BMSCs with EPO was significantly higher than that of BMSCs without EPO (P < 0.05); however, the level of MMP-2 was decreased gradually, especially in 16 U/mL EPO group. These indicate that EPO can increase the proliferative ability of BMSCs in a dose-dependent manner.

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