Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8842-8846.doi: 10.3969/j.issn.1673-8225.2011.47.025

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Chronic biocompatibility of chitosan/poly(lactic-co-glycolic acid) tissue engineered nerve transplanted into Beagles

Zhu Qi1, 2, Yang Yu-min2, Wu Jian2, Hu Wen2, Gu Xiao-song2   

  1. 1Department of Preventive Medicine, College of Public Health, Nantong University, Nantong  226019, Jiangsu Province, China
    2Key Laboratory of Neuroregeneraion of Jiangsu Province, Nantong University, Nantong  226001, Jiangsu Province, China
  • Received:2011-05-24 Revised:2011-07-05 Online:2011-11-19 Published:2011-11-19
  • Contact: Gu Xiao-song, Professor, Doctoral supervisor, Key Laboratory of Neuroregeneraion of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu Province, China neurongu@ public.nt.js.cn
  • About author:Zhu Qi★, Master, Lecturer, Department of Preventive Medicine, College of Public Health, Nantong University, Nantong 226019, Jiangsu Province, China; Key Laboratory of Neuroregeneraion of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu Province, China zgjsntzq@126.com
  • Supported by:

    the National 863 Program of China, No. 2006AA02A128*

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Abstract:

BACKGROUND: Short-term biocompatibility test within 2 months has showed that chitosan, polylactic acid and polyglycolic acid have no toxicity in rat peripheral nerve, which can be used as tissue engineered nerve materials.
OBJECTIVE: To evaluate the chronic biocompatibility of the chitosan/poly(lactic-co-glycolic acid) (PLGA) tissue engineered nerve transplanted into beagles.
METHODS: The tissue engineered nerve was prepared through inserting the PLGA filaments into chitosan conduits, and then transplanted into beagles bridging sciatic nerve 50 mm defect, and autologous nerve graft was taken as control at the same time.
RESULTS AND CONCLUSION: The status, appetite and activity of beagles which were transplanted with chitosan/PLGA tissue engineered nerve were good and the weight gain were similar to the autologous nerve graft group. Hematology and serum biochemical tests results after 2, 4, 6 months were also similar to the control group and there were no significant difference between two groups. Necrosis and degeneration was not found in the regenerated nerve as well as the surrounding tissues. And the tissue biopsies of the heart, liver, spleen, lung, kidney and other major organs were not abnormal. The chronic biocompatibility of the chitosan/PLGA tissue engineered nerve which was transplanted into Beagles for 6 months was good.

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