Abstract:

BACKGROUND: Currently, in the pharmacokinetic studies of the eye, the sample selection is often carried out in vitro, and there are many disadvantages.
OBJECTIVE: To develop a novel ocular microdialysis technology in conscious rabbit eyes, and to compare the difference of the pharmacokinetics of vancomycin after intravenous and intravitreal administration.
METHODS: Fifteen healthy and mature rabbits were used to prepare endophthalmitis models and were randomly divided into three groups (intravenous administration, intravitreal administration and intravenous+intravitreal administration) at 24 hours after microdialysis probe was implanted. Vitreous vancomycin concentration was detected by high performance liquid chromatography-ultraviolet (HPLC-UV).
RESULTS AND CONCLUSION: The vancomycin concentration in the intravenous administration group was much lower than the other two groups. At 72 hours after administration, the vancomycin concentrations in the intravitreal administration and intravenous+intravitreal administration groups reached a therapeutic concentration. It is indicated that microdialysis combined with HPLC-UV can continuously and in vivo detect the vancomycin concentration in the vitreous body, and single administration of vancomycin cannot reach a therapeutic concentration.