Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8825-8828.doi: 10.3969/j.issn.1673-8225.2011.47.021

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Prevention and treatment of post-traumatic orbital tissue adhesion by using chitosan membrane

Ren Ming-yu1, Zhao Hong2, Li Jing3   

  1. 1Department of Ophthalmology, Second Affiliated Hospital of Xingtai Medical College, Xingtai  054001, Hebei Province, China
    2Ophthalmology Hospital of Tianjin City, Tianjin  300020, China
    3Third Hospital of Xingtai City, Xingtai  054001, Hebei Province, China
  • Received:2011-05-19 Revised:2011-08-03 Online:2011-11-19 Published:2011-11-19
  • About author:Ren Ming-yu★, Master, Attending physician, Department of Ophthalmology, Second Affiliated Hospital of Xingtai Medical College, Xingtai 054001, Hebei Province, China rmingyu2007@ yahoo.com.cn
  • Supported by:

    the Science and Technology Foundation of Tianjin Health Bureau, No. 05ky28*

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Abstract:

BACKGROUND: The macromolecular biomaterial, chitosan membrane (CM) with low immunogenicity, can be resolved and generally used in the field of soft tissue adhesion. But the orbit included much subtle texture and owned the minor space, the application of CM in the orbit has not been general at present.
OBJECTIVE: To observe the influence and mechanism of CM in prevention and treatment of soft tissue adhesion after orbital trauma.
METHODS: A total of 10 rabbits with 20 orbits were randomly divided into two groups, namely, CM group and control group. The adhesion models of rabbits were established first, then the adhesion tissues were separated after two weeks, and CM about   15 mm×15 mm were implanted between the lesion and periost. The CM was not used in the control group.
RESULTS AND CONCLUSION: Four weeks after experiment, the adhesion degree was lower in the CM group than the control group (P < 0.01). The quantity of fibroblasts in unit area and absorbance value in unit area of collagen fibers were both significantly lower in the CM group than the control group (P < 0.01). Relative area of collagen fibers was also lower in the CM group as compared with the control group (P < 0.01). The structures of retina and optic nerve were not changed in histopathology. CM can inhibit the proliferation of fibroblasts, and decrease the synthesis of collagen fibers, which is effective on preventing adhesion after the orbital trauma. And it has no effect to the retina and optic nerve.

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