Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8801-8804.doi: 10.3969/j.issn.1673-8225.2011.47.015

Previous Articles     Next Articles

Preparation and biological safety of basic fibroblast growth factor/double-layered collagen composite

Wang Jian-hua1, Li Xue-min1, Tao Xiao-jun1, Chen Hui-juan1, Shi Jia-wei1, Liu Ling-rong1, Zhang Qi-qing1,2   

  1. 1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, the Key Laboratory of Biomedical Material of Tianjin, Tianjin  300192, China
    2Research Center of Biomedical Engineering, Xiamen University, Xiamen  361005, Fujian Province, China
  • Received:2011-07-05 Revised:2011-08-04 Online:2011-11-19 Published:2011-11-19
  • Contact: Zhang Qi-qing, Researcher, Doctoral supervisor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, the Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China; Research Center of Biomedical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@ xmu.edu.cn
  • About author:Wang Jian-hua☆, Doctor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, the Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China mwangjianhua@ yahoo.com.cn
  • Supported by:

    the Tackle Key Program of Tianjin Municipal Science and Technology Commission, No. 08ZCKFSF02100*; Doctoral Station Foundation, No. 20101106110042*

PDF

442

Abstract:

BACKGROUND: Collagen is a common used scaffold in tissue engineering, its specific molecular structure and biological activity are conducive to a variety of cell adhesion, proliferation and differentiation, and can speed up wound healing and degradation to provide sufficient space for new tissues.
OBJECTIVE: To prepare a kind of inhomogeneous double layered collagen composite incorporated with basic fibroblast growth factor (bFGF) loaded chitosan-heparin (CS-Hep) nanoparticles, and to evaluate its biological safety.
METHODS: The air-dried dense layer collagen membranes and freeze-dried loose layer collagen membrane were prepared respectively and then cross-linked by D-ribose (termed as CAM and CFM respectively). CS-Hep nanoparticles were prepared and then dropped on the surface of CFM. The wet CAM was laid on the surface of CFM with nanoparticles, and then air dried completely at 4 ℃. The obtained material was termed as bDM. Its physical and chemical properties were investigated. In addition, its bio-safety was also examined through acute systemic toxicity, pyrogen, hemolysis and cellular toxicity tests.
RESULTS AND CONCLUSION: The bFGF/double-layered collagen composite was made up of double layers with one dense layer (CAM) and another loose layer (CFM). As part of bDM, CS-Hep nanopartiles with irregular global shape were aligned in the scope of D-period structure of the inner surface of CFM; the bFGF/double-layered collagen composite had no systemic acute toxicity, no cytotoxicity and pyrogen reactions as well as no hemolytic effect. The bFGF/double-layered collagen composite is prepared and found to have good biocompatibility and safety. Moreover, this material conforms to the ISO 10993-1, and can be used as a basic scaffold material in tissue engineering.

CLC Number: