Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8630-8634.doi: 10.3969/j.issn.1673-8225.2011.46.019

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Angiogenesis and expression of fibroblastic growth factor mRNA and protein after needle-embedding therapy of Neiguan in miniature pigs with myocardial ischemia injury

Yang Xiao-fang1, Cui Jin1, Liu Xiao-yu2, Xu Zhao3, Zhang Xiao-shan1, Feng Lin1, Wang Xing-gui1, Qian Ning4   

  1. 1Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 2Department of Traditional Chinese Medicine, Tenth People’s Hospital of Tongji University, Shanghai  200072, China; 3Laboratory of Morphology and Function, School of Basic Medical Science, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 4Institute of Zoology, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China
  • Received:2011-05-19 Revised:2011-10-10 Online:2011-11-12 Published:2011-11-12
  • Contact: Cui Jin, Doctor, Professor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China cj1108@21cn.com
  • About author:Yang Xiao-fang☆, Doctor, Associate professor, Master’s supervisor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China yangxiaofang210@163.com
  • Supported by:

    the Guizhou Committee of Science and Technology, No. J [2007]2109*

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Abstract:

BACKGROUND: Angiogenesis and fibroblast growth factors are closely related to the repair of myocardial ischemia injury. Myocardial ischemia injury can be prevented and cured by acupuncturing Neiguan. It is unclear that whether the mechanism behind this is related to the angiogenesis and fibroblast growth factors.
OBJECTIVE: To explore the effect of needle-embedding therapy of Neiguan on the angiogenesis and expression of fibroblast growth factor gene and protein in miniature pigs after myocardial ischemia injury.
METHODS: A number of 32 miniature pigs were randomly divided into 4 groups. The myocardial ischemia injury model was established by ligating the left anterior descending coronary artery. The left anterior descending coronary artery of the sham-operated pigs was only threaded without ligation. Pigs in Neiguan group and Geshu group were treated with needle-embedding therapy of Neiguan and Geshu respectively after establishing the myocardial ischemia injury model. Model group and sham-operated group received no treatment.
RESULTS AND CONCLUSION: According to immunohistochemical staining, the capillary density of cardiac muscle in miniature pigs was lowered after coronary artery ligation (P < 0.05). The capillary density of cardiac muscle increased on the 7th day after needle-embedding therapy of Neiguan and Geshu (P < 0.05 or P < 0.01). Neiguan group preceded Geshu group (P < 0.05). According to real time PCR and western blot, the fibroblast growth factor mRNA and protein expression of myocardial tissue in miniature pigs were significantly improved after coronary artery ligation (P < 0.05 or P < 0.01). The fibroblast growth factor mRNA and protein expression of Neiguan group and Geshu group improved, and the effect of needle-embedding therapy of Neiguan was the most evident (P < 0.05). It reveals that needle-embedding therapy of Neiguan and Geshu can increase the fibroblast growth factor mRNA and protein expression of myocardial tissue, leading to an increase of cardiac muscle capillary density, therefore ameliorate the myocardial ischemia. And the needle-embedding therapy of Neiguan is better than that of Geshu.

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