Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8625-8629.doi: 10.3969/j.issn.1673-8225.2011.46.018

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Effects of Naoyian serum on transforming growth beta 1 mRNA expression in cultured rat cerebral microvascular endotheal cells with hypoxia

Wan Sai-ying1, Li Xing-qun2, Zhi Yi-hui2, Luo Yun2   

  1. 1Department of Neurology, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Foshan  528000, Guangdong Province, China
    2Department of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha  410008, Hunan Province, China
  • Received:2011-05-13 Revised:2011-07-29 Online:2011-11-12 Published:2011-11-12
  • Contact: Li Xing-qun, Professor, Doctoral supervisor, Department of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China xgwansy@163.com
  • About author:Wan Sai-ying☆, Doctor, Associate chief physician, Department of Neurology, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Foshan 528000, Guangdong Province, China
  • Supported by:

    a grant from State Administration of Traditional Chinese Medicine of China, No. 2000-J-B-03*

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Abstract:

BACKGROUND: Clinical and animal experiments have confirmed that Naoyian (NYA) serum can promote hematoma absorption during acute cerebral hemorrhage and subsequent neurological function recovery and improve patients’ living quality.
OBJECTIVE: To determine the effects of NYA serum on the expression of transforming growth β1 (TGF-β1) mRNA in cultured rat cerebral microvascular endothelial cells (RCMECs) with hypoxia.
METHODS: RCMECs were cultured with the culture medium consisting of 5% NYA serum or 5%normal serum in an anaerobic incubator for 18 hours so as to establish the hypoxia models. The cultured RCMECs were randomly divided into four groups: normal, model, normal serum and NYA serum. The viability of RCMECs was determined by MTT method. TGF-β1 mRNA expression was measured by reverse transcription-polymerase chain reaction (RT-PCR). 
RESULTS AND CONCLUSION: The cultured RCMECs exhibited damage after 18 hours of hypoxia injury. The number of surviving RCMECs in NYA serum group was significantly higher than that in the model and normal serum groups, as determined by MTT method. TGF-β1mRNA expression was increased by hypoxia in the cerebral microvascular endothelial cells. TGF-β1mRNA expression in the NYA serum group was significantly lower than that in the other groups (P < 0. 05). These findings suggest that NYA serum downregulating TGF-β1mRNA expression may be one of mechanisms by which NYA serum inhibits apoptosis of cerebral microvascular endothelial cells and then protects cerebral microvascular endothelial cells from hypoxia injury.

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