Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8621-8624.doi: 10.3969/j.issn.1673-8225.2011.46.017

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Comparison of growth and metabolism of cholangiocarcinoma cells in two-dimensional and three-dimensional culture systems

Li Lei1, Zhou Wei-jia1, Wang Jian-dong2, Ye Zhao-yang1, Zhou Yan1, Tan Wen-song1   

  1. 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai  200237, China
    2Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Received:2011-06-10 Revised:2011-09-20 Online:2011-11-12 Published:2011-11-12
  • Contact: Zhou Yan, Master, Associate professor, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China zhouyan@ecust.edu.cn
  • About author:Li Lei★, Master, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China lileifalcon@163.com
  • Supported by:

    the Natural Science Foundation of Shanghai, No. 10ZR1407100*; the National Foundation of Key Laboratory, No. 2060204*

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Abstract:

BACKGROUND: Two-dimensional (2D) culture model is a common tool for tumor research and drug screening in vitro; however, there are some limitations.
OBJECTIVE: To establish a three-dimensional (3D) culture model of cholangiocarcinoma cells and to compare the difference in cell growth and metabolism between 2D and 3D culture systems.
METHODS: Cholangiocarcinoma cell line QBC939 was cultured in 2D and Cytodex-3 or Cytopore-2 microcarrier-based 3D culture systems. Cell morphology was observed under inverted light microscopy. Total cell number, glucose and lactate concentrations were determined, and the kinetic constants of cell growth and metabolism were calculated.
RESULTS AND CONCLUSION: In comparison with 2D and Cytodex-3-based 3D culture systems, Cytopore-2-based 3D culture system achieved higher cell adhesion rate, higher cell density, and lower specific glucose consumption and specific lactate production rates (P < 0.05). In addition, cells were uniformly distributed in Cytopore-2 microcarriers. Therefore, Cytodex-3 or Cytopore-2 microcarriers can be used to establish cholangiocarcinoma 3D culture model, and Cytopore-2 3D culture system is better than Cytodex-3 system.

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